TABLE 2.

Primers used in this study

Strain construction and analysisNameSequence
ΔlacIZ::catlacI.ext-55′-GGGCGATCTGTTGCGCGAAG-3′
lacI.500-55′-CTTATCCTTTCACCGGGCAATG-3′
lacI.cat.L-35′-CTGCGAGTGATCTTCCGTCACAGGATTCACCACCCTGAATTGACTCT-3′
lacZ.cat.L-55′-GATGAGTGGCAGGGCGGGGCGTAATAATAACCGGGCAGGCCATGTC-3′
lacZ.500-35′-CGACCAAATTCGAAATTACTG-3′
lacZ.ext-35′-CAAACTGATTATTGATGGTGAACA-3′
lacI-lacZ intergenic regionLACZACTIV.A1.500-55′-CTCACTCGCAATCAAATTCAG-3′
lacZATG+100-35′-GGGGGATGTGCTGCAAGGCGATTAAG-3′
Random-primed PCRARB15′-GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT-3′
ARB25′-GGCCACGCGTCGACTAGTAC-3′
ARB65′-GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC-3′
Localization of MuMuR200-55′-AATTTAATCAGTATCGCTAC-3′
MuR100-55′-ATGTAATGAATAAAAAGCA-3′
MuL200-35′-GTTTTGAACGTTTTTTGAAG-3′
MuL100-35′-CTAAAATTTGCACTACAGGC-3′
Detection of MuMu1470bp.500-55′-GTTACTTTTCAAAATTTAAAC-3′
Mu1470bp.500-35′-CGCAGATAATCTGCAATCAG-3′
ΔMu1::apraMuL.apra.ext-55′-ACATTGGATTATTCGGGATC-3′
Mu.apra.500-55′-CGGGATCTGATGGGATTAGATTTG-3′
MuL.apra.L-35′-CAAGGGCTCCAAGGATCGGGCCTTGATAAACAAATTAACCACAACC-3′
tetR.MuR.apra.L-55′-CGCCAGTCGATTGGCTGAGCTCATGATCAGGACCGCTGCCGGAG-3′
tetR.apra.500-35′-CGGCGTTTCTGGCGCGTTTG-3′
tetR.apra.ext-35′-CCACATCATCTGTGGGAAACTC-3′
ΔMu2::zeotetA.zeo.ext-55′-ATATTTCGCTATTCTGGAGC-3′
tetA.zeo.500-55′-GGTGTTGACACCAAACGCAG-3′
tetA.zeo.L-35′-GTCAACACGTGCTCGGATCCAGAATCGTGGAAACGATAGGCCTATG-3′
glvB.zeo.L-55′-TTCGTGGCCGAGGAGCAGGACTGAACAGCTCGATAGCTTAATTAATTC-3′
glvB.500-35′-GCATGTCACAGATGTTGAGG-3′
glvB.ext-35′-GCGGGATATCGCCGACCACC-3′
ΔaphA::cat-FRTapha.Cmfrt.ext-55′-ACTACGAAATCGCCTACAGC-3′
apha.Cmfrt.TL-55′-TGAAATGGTGAGATTGCGTTGGCTGGCGGGGCACATTTCTTAATGTGTAGGCTGGAGCTGCTTC-3′
apha.Cmfrt.TL-35′-CCCGCCCAACGTCGATGCAGCCGACTACCTTACCTTCGATTAGTTCCTATTCCGAAGTTC-3′
apha.Cmfrt.ext-35′-GCATGAAAATGGCAAATAAC-3′
ΔrecA::kan-FRTrecA.ext-55′-GTGCTATCTTGTCCGGCATA-3′
recA.500-55′-GTTAAGTGAACAGGTTGGGC-3′
recA.500-35′-CAGAAAACGCTGGATCTTAAC-3′
recA.ext-35′-CTTCCATCAGATAGCCACGA-3′
ΔdapA::pir116-ermdapA.100-55′-TGAGTTGTTCTTAAGGAAAG-3′
dapA.100-35′-CAGGCAGCGAGTAATAAAACAAG-3′