Donor [relevant characteristics] (plasmid)^{a} | Recipient | Transconjugant frequency^{b} | % Mu transfer^{c} | % Lac^{+}^{c} |
---|---|---|---|---|

S17-1 λpir [ChRP4 pir^{+}] (pSC189) | MG1655 Δlac | 1.7 × 10^{−2} ± 5.5 × 10^{−3} | 63 ± 13 | 0.014 ± 0.009 |

S17-1 λpir [ChRP4 pir^{+}] (pSC189) with 10 mM sodium citrate | MG1655 Δlac with 10 mM sodium citrate | 3.4 × 10^{−3} ± 1.8 × 10^{−3} | 35 ± 34 | ND^{d} |

S17-1 λpir [ChRP4 pir^{+}] | MG1655 Δlac | ND | 61 ± 17^{e} | 0.55 ± 0.37^{e} |

SM10 λpir [ChRP4 pir^{+}] (pSC189-Cm) | MG1655-s Δlac | 6.3 × 10^{−2} ± 5.4 × 10^{−2} | 89 ± 10 | <0.001 |

ω7249 [ChRP4Δnicpir^{+}] (pSC189-Cm) | MG1655-s Δlac | 1.2 × 10^{−3} ± 3.2 × 10^{−4} | 93 ± 3 | <0.001 |

MFDpir [ChRP4 Δnic ΔMu pir^{+}] (pSC189-Cm) | MG1655-s Δlac | 2.0 × 10^{−3} ± 1.0 × 10^{−3} | <0.72 | <0.001 |

↵

*a*ChRP4, chromosomal integration of RP4-2-Tc::Mu.↵

*b*Expressed as the mean number ± standard deviation of transconjugants per recipient cell.↵

*c*Expressed as the percentage ± standard deviation of events in the transconjugant population. The percentage of Mu transfer was determined by PCR on 46 colonies per mating. The percentage of Lac^{+}colonies was determined by plating on X-Gal-containing agar plates. Three independent matings were done for each condition.↵

*d*ND, not determined.↵

*e*Expressed as the percentage ± standard deviation of events in the exconjugant recipient population.