TABLE 2.

Primers used for quantitative real-time RT-PCR

PrimerSequenceTargetUseReferencea
oSRB795′-ATACTCATCTTCGACATA-3′ybgEFirst-strand synthesis
oSRB805′-ACGATCATAAATGTATAGCTCCT-3′ybgEqPCR
oSRB825′-GATTAATAGATCACATTCA-3′ilvBFirst-strand synthesis
oSRB835′-ATTTACGATAAGCTATACAATTCA-3′ilvBqPCR
oSRB945′-AATCAATCATGGCATCAGCAA-3′ilvBqPCR
oSRB1005′-AATTAGTTGAGCTGGAGAAAGATT-3′ybgEqPCR
oSRB1185′-TATTGGCATGGACGATTT-3′ptbFirst-strand synthesis
oSRB1195′-ATGATGTCAGCTCGTTCAGTTTTTT-3′ptbqPCR
oSRB1205′-TGAAAGATTTAATCGGCAAAGCGT-3′ptbqPCR
oSRB1705′-TCTTGAAGCTCGTTTTCGTTGA-3′ilvDFirst-strand synthesis, qPCR
oSRB1715′-TCGCAATGGGGCATATCGGTAT-3′ilvDqPCR
rRNA16SF5′-GGGTGATCGGCCACAC-3′rrnAqPCR47
rRNA16SR5′-CCCCAGTTTCCAATGACC-3′rrnAFirst-strand synthesis, qPCR47
  • a Unless otherwise noted, primers were designed during this study.