TABLE 3.

Distribution of LSPs across M. aviuma

SpeciesStrainSouthern blotNon map specificM. avium subsp. paratuberculosis specificM. avium subsp. paratuberculosis lineage specific
LSPP 1LSPP 3LSPP 5LSPP 6LSPP 7eLSPP 8fLSPP 10LSPP 13LSPP 17LSPP 2bLSPP 4LSPP 11LSPP 12LSPP 14LSPP 15LSPP 16cLSPA 8GPLdLSPA 4-IIMAV 14LSPA 18LSPA 20Deletion 2VA 15LSPA 11
M. avium subsp.
    hominissuis104Yes-ii+104+++++++
MB063764Yes+++i+++++++++
ATCC 49601Yes+i++++ii+104+++++++
76102Yes+i+++i+i+104+++++++
108No+i+++i+i+104+++++++
DVYes+i+i+104+++++++
28132Yes+i+i+104+++++++
MB064490Yes+i+i+104+++++++
2000-325No+++++iNDi+++ND+++++
HMC36ANo++++i+++++++++
    aviumATCC 25291TYes++++iii+++i+++++
ATCC 35713Yes++++iii+++i+++++
ATCC 15796Yes++++iii+++i+++i+
    silvaticumATCC 49884TYes++++iii+++i+++i+
9800851No++++iii+++i+++i+
    paratuberculosis, cattle genotypeMAP K-10Yes++++++++++++++++K-10++++
316FYes++++++++++++++++K-10++++
MAP 119Yes++++++++++++++++K-10++++
7296Yes++++++++++++++++K-10++++
    paratuberculosis, sheep genotype6756Yes++++++++++++++++++++++
85/14Yes++++++++++++++/-++++++i+
LN20Yes+++++++++++++++++++++
P465Yes++++++++++++++++++++++
M. intracellularegATCC 13950TNo++MIhMI++++++++
  • a +, Positive by multiplex PCR, LSP present; −, negative by multiplex PCR, LSP absent; i, internal target not detected, LSP absent; +/−, see the text (strain-specific deletion after acquisition of LSPP14). For the non-M. avium subsp. paratuberculosis-specific LSPs, the location previously described was absent in strain 104 (17). For the M. avium subsp. paratuberculosis-specific and M. avium subsp. paratuberculosis-lineage-specific LSPs, the locations can be found in Table S2 in the supplemental material. ND, not determined.

  • b In negative strains, LSPP2 is replaced by the LSPA21 polymorphism.

  • c In negative strains, LSPP16 is replaced by the LSPA24 polymorphism.

  • d In negative strains, GPL conformation can match that found in M. avium subsp. paratuberculosis strain K-10 or M. avium subsp. hominissuis strain 104.

  • e In negative strains, LSPP7 is replaced by the MAV15 polymorphism.

  • f In negative strains, LSPP8 is replaced by the LSPA15 polymorphism, except for strain HMC36A, which exhibits a unique polymorphism.

  • g In negative strains, some LSPs are replaced by M. intracellulare-specific polymorphisms.

  • h MI, M. intracellulare.