TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Source or reference
Strains
        V. vulnificus
        ATCC 29307Clinical isolate; virulent14
        ARATCC 29307 but spontaneous rifampin resistance26
        HY101AR but ΔcpdA20
        KP301AR but Δcya20
        KC74ATCC 29307 but crp::nptI14
    E. coli
        DH5α(φ80 lacZΔM15) recA1 endA1 gyrA96 relA1 thi-1 hsdR17(rK mK) supE44 deoR Δ(lacZYA-argF)U169Laboratory collection
        SM10λpirthi-1 thr leu tonA lacY supE recA::Rp4-2-Tc::Muλpir; OriT of RP4; Kmr30
        JM109endA1 recA1 gyrA96 thi-1 hsdR17(rK mK) relA1 supE44 Δ(lac-proAB) [F′ traD3 6proAB lacIqZΔM15]Promega
Plasmids
    pUC19Cloning vector; lacZ Apr34
    pINE45pUC19 with 1.72-kb Sau3AI fragment of V. vulnificus DNA containing the cpdA geneThis study
    pINE45-1pUC19 with 4.3-kb Sau3AI fragment of V. vulnificus DNA containing the cpdA geneThis study
    pLAFR5IncP Tcr; derivative of pLAFR3 containing double cos cassettes16
    pHS51pLAFR5 containing EcoRI and HindIII fragment of pINE45This study
    pQE30Expression vector; AprQiagen
    pQE30-cpdApQE30 with V. vulnificus cpdAThis study
    pHK0011Transcriptional fusion plasmid with promoterless luxAB; Tcr8
    pSMK-cpdA-1pHK0011 with cpdA upstream region (positions −1521 to +41 relative to cpdA IC)This study
    pSMK-cpdA2pHK0011 with cpdA upstream region (positions −1178 to −1051 relative to cpdA IC)This study
    pSMK-cpdA3pHK0011 with cpdA upstream region (positions −1163 to −1051 relative to cpdA IC)This study
    pGEM-11zf(+)General cloning vector; AprPromega
    pGEM-11zf(+)-cpdA2mtpGEM-11zf(+) with the mutagenized sequence for the putative CRP-binding site of cpdA regulatory regionThis study
    pSMK-cpdA2mtpSMK-cpdA2 but mutagenized CRP-binding siteThis study