TABLE 1.

Strains and plasmids

Strain or plasmidGenotype/featuresReference or source
P. aeruginosa strainsa
    PAKWild type strain51
    AJDP65PA0943::mariner-GmrThis study
    AJDP69rpoN::mariner-GmrThis study
    AJDP124PA4068::mariner-GmrThis study
    AJDP168PA4069::mariner-GmrThis study
    AJDP250truB::mariner-GmrThis study
    AJDP267trkA::pAJD1This study
    AJDP270PA3731::pAJD1This study
    AJDP279ΔalgUThis study
    AJDP388ΔPA0943This study
    AJDP392ΔPA4068This study
    AJDP419ΔPA4069This study
    AJDP422Δ(PA4069-PA4068)This study
    AJDP504ΔpurMNThis study
    AJDP511attB::[ΔPA4069-PA4068+]This study
    AJDP512attB::[PA4069+]This study
    AJDP513attB::[PA4069+-PA4068+]This study
    AJDP514ΔPA4068 attB::[ΔPA4069-PA4068+]This study
    AJDP515ΔPA4068 attB::[PA4069+-PA4068+]This study
    AJDP518ΔPA4069 attB::[PA4069+]This study
    AJDP519ΔPA4069 attB::[PA4069+-PA4068+]This study
    AJDP522Δ(PA4069-PA4068) attB::[ΔPA4069-PA4068+]This study
    AJDP523Δ(PA4069-PA4068) attB::[PA4069+]This study
    AJDP524Δ(PA4069-PA4068) attB::[PA4069+-PA4068+]This study
    AJDP553attB::[ΔpurMN-PA0943+]This study
    AJDP556ΔPA0943 attB::[ΔpurMN-PA0943+]This study
    AJDP605Δ(xcpZ-Q) Δ(xphA-xqhA)This study
Plasmids
    pBTK30mariner-Gmr delivery plasmid, R6K ori17
    pEX18ApAmpr, pMB1 ori, oriT, sacB+22
    pFLP2Ampr, pMB1 ori, pRO1600 ori, oriT, sacB+, FLP+22
    pJN105Gmr, araBp expression vector, pBBR ori39
    pmini-CTX-T7Tetr, pMB1 ori, oriT, int+, attP+23
    pVLT35Smr, Spr, tacp expression vector, RSF1010 ori12
    pAJD1Smr, Spr, R6K oriThis study
    pAJD942tacp-xcpQ in pVLT35This study
    pAJD1164tacp-oprF in pVLT35This study
    pAJD1365araBp-pspBC in pJN105This study
  • a All P. aeruginosa strains are derivatives of strain PAK.