TABLE 1.

Primers and oligonucleotide sequences used for the construction of S. coelicolor NrdR mutants

PrimerSequence (5′-3′)a
NrdR_V48A_FORGCTCGCTCATGGCTGTGAAGCGGTC
NrdR_V48A_REVGACCGCTTCACAGCCATGAGCGAGC
NrdR_K50A_FORCATGGTGGTGGCTCGGTCCGGGGTC
NrdR_K50A_REVGACCCCGGACCGAGCCACCACCATG
NrdR_R51A_FORATGGTGGTGAAGGCGTCCGGGGTCA
NrdR_R51A_REVTGACCCGGACGCCTTCACCACCAT
NrdR_E56A_FORCGGGGTCACCGCTCCGTTCAGCCGC
NrdR_E56A_REVGCGGCTGAACGGAGCGGTGACCCCG
NrdR_K62A_FORTCAGCCGCACCGCTGTGATCAACGG
NrdR_K62A_REVCCGTTGATCACAGCGGTGCGGCTGA
NrdR_Y121A_FORCCTCGTCGCCGCTCTGCGATTCGCC
NrdR_Y121A_REVGGCGAATCGCAGAGCGGCGACGAGG
NrdR_Y128A_FORTTCGCCTCCGTCGCCCGGGCGTTCG
NrdR_Y128A_REVCGAACGCCCGGGCGACGGAGGCGAA
NrdR_V63A_FORTTCAGCCGCACCAAGGCCATCAACGGTGTGCG
NrdR_V63A_REVCGCACACCGTTGATGGCCTTGGTGCGGCTGAAC
  • a Underlining indicates mutant nucleotides.