TABLE 3.

In vivo effects of TtgV and its mutant variants on the expression of ttgD (pMPD) and ttgG (pMPG) promoters fused to ′lacZa

StrainPresence of 1-naphtholβ-Galactosidase expression (Miller units)Mutant group
TIEVT(pMPD)600 ± 60Not relevant
+600 ± 100Not relevant
TIEVT(pMPG)1,900 ± 160Not relevant
+2,100 ± 160Not relevant
TIEVT(pBBRN::ttgV, pMPD)30 ± 5Not relevant
+550 ± 120Not relevant
TIEVT(pBBRN::ttgV, pMPG)750 ± 40Not relevant
+1,850 ± 110Not relevant
TIEVT(pBBRN-Q51A, pMPD)10 ± 51
+150 ± 501
TIEVT(pBBRN-Q51A, pMPG)100 ± 201
+2,400 ± 2101
T1EVT(pBBRN-E58A, pMPD)10 ± 101
+130 ± 51
T1EVT(pBBRN-E58A, pMPG)30 ± 201
+1,560 ± 801
TIEVT(pBBRN-R47A, pMPD)600 ± 802
+900 ± 302
TIEVT(pBBRN-R47A, pMPG)2,500 ± 2002
+2,800 ± 3902
TIEVT(pBBRN-V50A, pMPD)50 ± 53
+750 ± 603
TIEVT(pBBRN-V50A, pMPG)2,250 ± 1503
+2,600 ± 4703
TIEVT(pBBRN-E60A, pMPD)100 ± 103
+800 ± 1003
TIEVT(pBBRN-E60A, pMPG)2,200 ± 3003
+2,800 ± 5803
  • a Pseudomonas putida strains deficient in ttgV and ttgT were transformed with plasmid pMPD1 or pANA96 and pBBRN::ttgV or its mutant derivatives. Cells were grown on LB medium as described in Materials and Methods with rifampin, tetracycline, and kanamycin in the absence (−) and in the presence (+) of 1 mM 1-naphthol. β-Galactosidase activity was assayed in permeabilized cells as described in Materials and Methods.