TABLE 3.

Effects of deletions 3′ to the transcriptional start site on temperature regulation of PR3

StrainMean β-Galactosidase activity (Miller units) ± SEMa
37°C20°C
P90C(pRS415)78 ± 774 ± 9
P90C(pRSC)24,643 ± 15724,800 ± 164
P90C(pRSA)1,044 ± 8599 ± 8
P90C(pRSA-1)577 ± 2978 ± 5.5
P90C(pRSA-2)1,375 ± 90212 ± 15
P90C(pRSA-3)2,211 ± 79113 ± 9
P90C(pRSA-4)2,907 ± 81150 ± 10
P90C(pRSA-5)7,886 ± 87424 ± 23
P90C(pRSA-6)11,000 ± 85587 ± 28
P90C(pRSA-11)12,954 ± 952,800 ± 75
P90C(pRSA-12)18,000 ± 12317,056 ± 216
P90C(pRSA-17)20,000 ± 11821,000 ± 230
  • a β-Galactosidase activity of P90C bearing either the promoter probe plasmid pRS415 or derivatives was assayed at 37°C and 20°C. Plasmid pRSA, pRSC, and pRSA1-17 are described in Fig. 2 and Table 1. Data are the means of three independent experiments performed in triplicate.