TABLE 2.

Expression from PR3 at 37°C and 20°C

StrainMean β-Galactosidase activity (Miller units) ± SEMa
37°C20°C
P90C(pRS415)132 ± 17100 ± 7
P90C(pRSA)1319 ± 90120 ± 9
P90C(pRSB)140 ± 15158 ± 15
P90C(pRSC)10,326 ± 25212,000 ± 190
  • a β-Galactosidase activity of P90C bearing either the promoter probe plasmid pRS415 or derivatives was assayed at 37°C and 20°C. Plasmids pRSA, pRSB, and pRSC are described in Fig. 1 and Table 1. Data are the means of three independent experiments performed in triplicate.