TABLE 1.

Bacterial strains and plasmids used in this study

E. coli strain or plasmidRelevant genotypeSource or reference
    BL-21F dcm ompT hsdS(rB mB ) gal Stratagene
    MC4100hns hns::kan 27
    MG1655slyA::kan slyA::kan J. Green
    MG1655slyA::Cmr slyA::Cmr Peng Xue
    P90CF ara Δ(lac pro) thi-1 29
    P90Chns::kan P90C × P1(MC4100hns)This study
    P90CslyA::kan P90C × P1(MG1655slyA::kan)This study
    P90Chns::kan slyA::Cmr P90Chns::kan × P1(MG1655slyA::Cmr)This study
Plasmids
    pACYC184Cloning vector 5
    pACYC184-SlyApACYC184 containing slyA coding region and promoter 6
    pET22bHis fusion expression vectorNovagen
    pET22b-H-NSpET22b, hns + 6
    pGEX-6P-1GST fusion expression vectorAmersham Biosciences
    pRS415 lacZ promoter-probe vector 29
    pBluescriptSK+Cloning vectorStratagene
    pRSApRS415+PR3 (−320 to +741)This study
    pRSBpRS415 + the sequences (+1 to +741) 3′ to PR3This study
    pRSCpRS415+PR3 (−320 to +1)This study
    pRSA-156-bp deletion of pRSA (Fig. 2)This study
    pRSA-2107-bp deletion of pRSA (Fig. 2)This study
    pRSA-3216-bp deletion of pRSA (Fig. 2)This study
    pRSA-4276-bp deletion of pRSA (Fig. 2)This study
    pRSA-5299-bp deletion of pRSA (Fig. 2)This study
    pRSA-6361-bp deletion of pRSA (Fig. 2)This study
    pRSA-11456-bp deletion of pRSA (Fig. 2)This study
    pRSA-12658-bp deletion of pRSA (Fig. 2)This study
    pRSA-17706-bp deletion of pRSA (Fig. 2)This study
    pBlue-CpSK+ with a cloned 356-bp PCR product (−320 to +36)This study
    pBlue-HpSK+ with a cloned 245-bp PCR product (+1 to +246)This study