TABLE 4.

Genes with decreased expression in the serovar Typhimurium igaA1 mutant versus the wild-type strain

Systematic gene nameGeneProductLog2 ratio for the indicated comparisonaReverence(s) if the product was previously assigned to the Rcs regulon
igaA1 mutant vs WTigaA1 rcsB mutant vs WTrcsB mutant vs WTigaA1 mutant vs igaA1 rcsA mutantigaA1 rcsA mutant vs WT
PSLT039spvBHydrophilic protein−4.0−4.5−4.2−0.9−3.1
PSLT040spvAOuter membrane protein−5.1−5.2−4.6−1.8−3.2
STM0462glnKNitrogen regulatory protein P-II 2−2.60.60.7−2.8−0.04
STM0829glnPGlutamine ABC transporter permease component−2.50.30.1−1.7−0.9
STM1172flgMAntiflia factor−2.9−1.6−1.20.4−3.555
STM1174flgBFlagellar basal body rod protein−3.0−0.9−1.01.5−5.020, 55
STM1175flgCFlagellar basal body rod protein−3.9−2.1−2.01.6−5.846, 55
STM1176flgDFlagellar basal body rod modification protein−3.6−1.9−1.81.6−5.520, 55
STM1177flgEFlagellar hook protein−3.5−1.8−1.41.2−5.155
STM1178flgFCell-proximal portion of basal body rod−3.1−1.7−1.71.8−5.355
STM1179flgGFlagellar basal body rod protein−3.4−1.5−1.50.4−3.946, 55
STM1181flgIFlagellar P-ring protein precursor−2.5−1.3−1.71.3−4.046, 55
STM1182flgJFlagellar biosynthesis protein−2.5−1.2−0.60.2−2.855
STM1183flgKFlagellar hook-associated protein−3.1−1.5−1.51.1−4.455
STM1916cheYChemotaxis regulator−3.0−1.5−1.50.8−4.055
STM1917cheBChemotaxis-specific methylesterase−2.5−1.5−1.21.1−3.955
STM1919cheMMethyl-accepting chemotaxis protein II−3.1−1.4−1.4−0.06−3.255
STM1921cheAChemotaxis sensory histidine protein kinase−2.7−1.0−1.4−0.07−2.855
STM1922motBFlagellar motor protein−2.2−1.2−0.91.4−3.855
STM1956fliAFlagellar biosynthesis sigma factor flia−2.7−1.7−1.21.2−4.155
STM1959fliCFlagellar biosynthesis protein−3.1−1.3−1.51.9−5.355
STM1960fliDFlagellar hook-associated protein−3.2−1.2−0.80.3−3.855
STM1961fliSFlagellar protein flis−2.2−0.8−0.90.7−3.255
STM1962fliTPossible flid export chaperone−2.3−0.7−1.10.8−3.355
STM1970fliGFlagellar motor protein−2.2−1.5−0.80.06−2.555
STM1971fliHFlagellar assembly protein−2.5−1.2−0.90.09−2.755
STM1973fliJFlagellar protein−2.3−1.3−1.40.2−2.655
STM1974fliKFlagellar hook length control protein−2.1−1.4−1.20.1−2.455
STM1975fliLFlagellar biosynthesis protein−2.3−1.3−1.40.1−2.555
STM2259napAPeriplasmic nitrate reductase−3.70.70.8−0.4−3.3
STM2260napDPeriplasmic nitrate reductase−2.70.60.20.1−2.9
STM2283glpTSn-glycerol-3-phosphate transport protein−2.2−0.05−0.20.2−2.323
STM2284glpASn-glycerol-3-phosphate dehydrogenase large subunit−2.80.70.70.2−3.1
STM2771fljBFlagellar biosynthesis protein−3.1−1.3−1.50.9−2.055
STM3274yhbUPutative protease−2.30.80.2−0.6−1.5
STM3474nirBNitrite reductase large subunit−2.50.60.20.4−3.1
STM4229malEPeriplasmic maltose-binding protein−2.4−1.7−1.4−0.8−1.746
STM4231lamBMaltoporin precursor−2.7−2.1−1.6−0.5−2.346
  • a Each gene was represented by a single 70-mer oligonucleotide spotted in two separate sections of a microarray slide (see the supplemental material for details). For the statistical analyses, the two spots were considered different entities. The table lists only those genes identified in the comparison of the igaA1 mutant and the wild type, both of whose P values were ≤0.05 and whose two log2 ratios were ≤2. For simplification of this table, only the average of the two log2 ratios for each gene is shown (see Table S2 in the supplemental material for a complete list of log2 ratios and P values).

  • b No reference indicates no previous assignment.