Apparent viability of superdormant spores prepared with different nutrient germinantsb

Species/strainGerminantApparent viability (%)a
Without Ca-DPAWith Ca-DPA
B. subtilis PS533 (wild type)Valine2395
B. subtilis FB22 (ΔgerA gerBA*)Asparagine-glucose1397
B. megaterium (wild type)Glucose975
  • a Apparent viability is expressed relative to that of initial wild-type spores without Ca-DPA treatment.

  • b Superdormant spores were isolated using normal nutrient germinant levels, as described in Materials and Methods, and apparent spore viability was assessed on LB plates relative to the OD600 values of spore suspensions, as described in Materials and Methods.