TABLE 2.

Primers used for gene deletion

PrimerSequenceaApplication
FW relA UPCGATTATCTTGGCATGCCCAGAGGAAGrelA deletion
RV relA UPCCGCTGGGCCGATATCTATTTCTTCTTTTGrelA deletion
FW relA DOWNGAACCAATGGGGATATCTTAAAAGTGAGrelA deletion
RV relA DOWNGTGGTATCGCGCGGCCGCGAGATATTACrelA deletion
FW relQ UPCCGTCGTTTAATCTAGATAATCGGAATATGrelQ deletion
RV relQ UPGATCCCTCAATAAGCTTGAATTTCATTCrelQ deletion
FW relQ DOWNATAAGCAAATTAAGCTTGAAACACCAGrelQ deletion
RV relQ DOWNCGTCATTTTAACGGATCCGCCAGTTTGTArelQ deletion
  • a The underlined bases correspond to restriction sites included to aid in the subsequent cloning of the PCR products.