TABLE 4.

Succinate semialdehyde dehydrogenase activities of gabD genes when overexpressed in R. leguminosarum bv. viciae 3841

StrainOverexpressed geneaSSDH activitybNAD+/ NADP+ SSDH activity ratio
NAD+-dependentNADP+-dependent
RU3845 gabD1 137 (±4)174 (±5)0.8
RU3846 gabD2 337 (±11)65 (±4)5.2
RU3847 gabD3 55 (±13)38 (±8)1.4
RU1816c gabD4 189613.1
RU3849 gabD5 139 (±15)21 (±1)6.7
RU3850c gabD6 670.9
R. leguminosarum bv. viciae 3841None7 (±3)9 (±2)0.8
  • a Overexpressed genes were cloned in pRK415 and expressed from the lacZ promoter, except for gabD4, which was expressed from its native chromosomal promoter.

  • b Enzyme activity is given in nmol min−1 mg protein−1. The mean and standard error of the mean (shown in parentheses) of ≥3 experiments are shown.

  • c n = 2.