TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristicsaReference or source
Strains
    Sinorhizobium meliloti
        1021SU47 str-21 (wild type), Smr 21
        10K1K1021 (Δkup1::Km), Smr Kmr This work
        10K2SS1021 (Δkup2::Sm/Sp), Smr Spr This work
        10tAG1021 (ΔtrkA::Gm), Smr Gmr This work
        10KdpSS1021 (ΔkdpA::Sm/Sp), Smr Spr This work
        10K2K110K2SS (Δkup1::Km), Smr Spr Kmr This work
        10tAK110tAG (Δkup1::Km), Smr Gmr Kmr This work
        10tAK210tAG (Δkup2::Sm/Sp), Smr Gmr Spr This work
        10tAKdp10tAG (ΔkdpA::Sm/Sp), Smr Gmr Spr This work
        10tAK2110tAK2 (Δkup1::Km), Smr Gmr Spr Kmr This work
        10tAKK10tAKdp (Δkup1::Km), Smr Gmr Spr Kmr This work
    Escherichia coli
        DH5α supE44 ΔlacU169 φ80dlacZΔM15 recA1 endA1 gyrA96 thi-1 relA1 hsdR17 Bethesda Research Lab
        S17.1 thi pro recA hsdR hsdM; Rp4Tc::Mu; Km::Tn7; Tpr Smr Spr 32
Plasmids
    pBluescript II KSCloning vector; Apr Stratagene
    pGEM-T easyPCR cloning vector; Apr Promega
    pHP45ΩpBR322 derivative with a streptomycin/spectinomycin resistance cassette; Apr Smr Spr 9
    pHP45Ω-KmpBR322 derivative with a kanamycin resistance cassette; Apr Kmr 9
    pMS255pUC8 derivative with a gentamicin resistance cassette; Apr Gmr 3
    pK18mobsacB Suicide plasmid; Mob+, sacB; Kmr 31
    pSUP202Pol4Suicide plasmid; Tcr 11
    pGUS3 pnfeD::gusA translational fusion in pBI101 (Clontech); Kmr 14
    pJB3Tc19IncP cloning vector; Tcr Apr 5
    pJB3K1pJB3Tc19 derivative containing a PCR-amplified fragment including the kup1 gene from S. meliloti 1021; Tcr Apr This work
    pJB3tApJB3Tc19 derivative containing a PCR-amplified fragment including the trkA gene from S. meliloti 1021; Tcr Apr This work
  • a Smr, streptomycin resistance; Kmr, kanamycin resistance; Spr, spectinomycin resistance; Gmr, gentamicin resistance; Tpr, trimethoprim resistance; Apr, ampicillin resistance; Tcr, tetracycline resistance.