TABLE 2.

Fatty acid profiles of Shewanella strains grown under different conditionsa

StrainCulture conditionsbMean % (by weight) fatty acid ± SD BCFA/SC FA ratiocUFA/SFA ratiod
Iso-13:0Iso-15:016:118:120:5
WP3
    Untreated20°C9.3 ± 2.512.3 ± 1.522.9 ± 3.88.2 ± 1.76.5 ± 1.10.30.6
4°C23.3 ± 4.426.2 ± 3.517.7 ± 1.96.5 ± 1.014.1 ± 1.81.070.62
0.1 MPa11.2 ± 2.49.0 ± 1.67.2 ± 0.913.6 ± 1.13.2 ± 0.50.250.32
20 MPa15.3 ± 1.114.8 ± 1.92.9 ± 0.49.0 ± 1.29.1 ± 1.30.430.27
    Treatede 20°C31.8 ± 4.89.4 ± 1.39.0 ± 1.107.1 ± 0.90.70.19
4°C51.9 ± 5.16.3 ± 1.07.3 ± 1.1014.4 ± 1.81.390.28
0.1 MPa22.3 ± 4.46.1 ± 1.34.5 ± 0.703.7 ± 0.70.550.09
20 MPa27.6 ± 4.98.2 ± 2.21.9 ± 0.309.8 ± 1.60.850.13
    WP3ΔEPA 20°C9.4 ± 1.111.6 ± 0.928.9 ± 3.39.6 ± 0.900.310.63
4°C26.7 ± 3.834.7 ± 3.418.7 ± 2.56.9 ± 0.501.390.34
0.1 MPa10 ± 1.28.7 ± 1.312.9 ± 2.016.9 ± 2.400.230.42
20 MPa14.3 ± 2.212.7 ± 1.95.5 ± 1.111.5 ± 1.900.370.20
    WP3ΔLIV 20°C8 ± 1.09.3 ± 1.529.4 ± 3.310.8 ± 1.97.4 ± 0.80.210.91
4°C12.5 ± 1.712.3 ± 0.931.7 ± 1.610.7 ± 1.513.6 ± 1.60.331.27
0.1 MPa10.2 ± 1.59.1 ± 0.88.4 ± 1.712.0 ± 1.83.9 ± 0.60.230.32
20 MPa11.1 ± 1.610.3 ± 1.84.4 ± 1.011.0 ± 0.910.4 ± 1.30.270.35
  • a The data represent values derived from triplicate samples harvested in the early stationary phase of growth.

  • b 20 and 4°C cultures were grown aerobically in 2216E marine medium(with 0.3 mg/liter [12C]leucine) at 0.1 MPa; 0.1- and 20-MPa cultures were grown at 20°C in 2216E marine medium(with 0.3 mg/liter [12C]leucine).

  • c BCFA, iso-13:0, iso-15:0, and iso-17:0; SCFA, 12:0, 13:0, 14:0, 15:0, 16:0, 16:1, 18:0, 18:1, and 20:5.

  • d UFA, 16:1, 18:1, and 20:5; SFA, 12:0, iso-13:0, 13:0, 14:0, iso-15:0, 15:0, 16:0, iso-17:0, and 18:0.

  • e Cerulenin was used at 10 mg/ml.