TABLE 1.

Bacterial strains, phage, and plasmids used in this study

Strain, phage, or plasmidRelevant genotype or characteristicsReference or source
E. coli K-12 strains
    MG1655F λMichael Cashel
    CF7789MG1655 Δ lacI-Z(MluI)Michael Cashel
    KSA712CF7789 Δ (λ att-lom)::bla φ(csrA′-′lacZ)1 (hyb) Ampr KansThis study
    AAECO72MG1655 Δ fimB-HIan Blomfield
    DHB6521F λInch1(Kanrlac(MS265) mel NalrsupF58 (=suIII+) 11
    JM101 supE thiΔ (lac-proAB)[F′ traD36 proAB lacIqZΔM115] 44
    MC4100F Δ(argF-lac)U169 rpsL relA flhD deoC ptsF rbsR 23
    W3110FmcrA mcrB IN(rrnD-rrnE)1 λ 44
    RG1-BMG1655 csrB::cam (a precise deletion of csrB) 22
    MM5057Δ motB urvC-279::Tn10Mike Manson
    MHR 204MC4100 csgA2::Tn105 23
    RH106MC4100 rpoS::Tn10R. Hengge-Aronis
    SG20043MC4100 cpsE::Tn10Valerie Stout
    TK821MC4100 ompR331::Tn10 44
    TR1-5 or TRa csrA::kanR40
    glgAb glgA::kanRThis study
    DJ1MG1655 csgA2::Tn105This study
    DJ2TRMG1655 csgA2::Tn105This study
    DJ3MG1655 cpsE::Tn10This study
    DJ4TRMG cpsE::Tn10This study
    DJ6TRAAECO72This study
    DJ7AAECO72 csgA2::Tn105This study
    DJ8TRAAECO72 csgA2::Tn105This study
    DJ9AAECO72 cpsE::Tn10This study
    DJ10TRAAECO72 cpsE::Tn10This study
    DJ11MG1655 csgA2::Tn105 cpsE::Tn10This study
    DJ12TR MG1655 csgA2::Tn105 cpsE::Tn10This study
    DJ13AAECO72 csgA2::Tn105 cpsE::Tn10This study
    DJ14TRAAECO72 csgA2::Tn105 cpsE::Tn10This study
    DJ21MG1655 ΔmotB uvrC-279::Tn10This study
    DJ22AAECO72 ΔmotB uvrC-279::Tn10This study
    DJ24TR ΔmotB uvrC-279::Tn10This study
    DJ25TRAAECO72 ΔmotB uvrC 279::Tn10This study
    DJ30MG1655 rpoS::Tn10This study
    DJ31TR MG1655 rpoS::Tn10This study
    DJ32MG1655 rpoS::Tn10 csgA2::Tn105This study
    DJ33TR MG1655 rpoS::Tn10 csgA2::Tn105This study
    DJ34AAECO72 rpoS::Tn10This study
    DJ35TR AAECO72 rpoS::Tn10This study
    DJ36AAECO72 csgA2::Tn105 rpoS::Tn10This study
    DJ37TRAAECO72 csgA2::Tn105 rpoS::Tn10This study
    DJ40MG1655 ompR333::Tn10This study
    DJ41TR MG1655 ompR333::Tn10This study
    DJ42MG1655 ompR333::Tn10 csgA2::Tn105This study
    DJ43TR MG1655 ompR333::Tn10 csgA2::Tn105This study
    DJ44AAECO72 ompR333::Tn10This study
    DJ45TR AAECO72 ompR333::Tn10This study
    DJ46AAECO72 ompR333::Tn10 csgA2::Tn105This study
    DJ47TR AAECO72 ompR333::Tn10 csgA2::Tn105This study
Clinical strains
    C. freundii P5 24
    E. coli P18 24
    E. coli O157:H7 EF302 26
    S. enterica serovar Typhimurium ATCC 14028 4
Bacteriophages
    P1virStrictly lytic P1; forms clear plaquesCarol Gross
    λInCh1For chromosomal integration of genes11
Plasmids
    pUC19Cloning vector, Ampr 44
    pBR322Cloning vector, Ampr Tetr 44
    pCSR10 E. coli csrA gene in pUC19, Ampr 40
    pSTCSR5 S. enterica serovar Typhimurium csrA in pUC19, GenBank accession no. 276860 , AmprThis study
    pCSRH6-19Produces recombinant CsrA under IPTG induction, Ampr 27
    pCSRB-SFMinimal csrB in pUC18, Ampr 27
    pOP12Contains asd-glgCAP′ in pBR322 Tetr 25
    pOP245 glgA in pBR322, Tetr 25
    pJF02 glgP in pUC19, Ampr 51
    pMLB1034For construction of ′lacZ translational fusions, Ampr 41
    pTR151P1 PstI subclone from pTR151, csrA::kanR allele in pUC19, Ampr 40
  • a Indicates that the wild-type csrA allele has been replaced by csrA::kanR.

  • b Strains names with the suffix glgA contained a glgA::kanR insertion.