TABLE 1.

Bacterial strains, phage, and plasmids used in this study

Strain, phage, or plasmidRelevant genotype or characteristicsReference or source
E. coli K-12 strains
    MG1655F λMichael Cashel
    CF7789MG1655 Δ lacI-Z(MluI)Michael Cashel
    KSA712CF7789 Δ (λ att-lom)::bla φ(csrA′-′lacZ)1 (hyb) Ampr KansThis study
    AAECO72MG1655 Δ fimB-HIan Blomfield
    DHB6521F λInch1(Kanrlac(MS265) mel Nalr supF58 (=suIII+)11
    JM101supE thiΔ (lac-proAB)[F′ traD36 proAB lacIqZΔM115]44
    MC4100F Δ(argF-lac)U169 rpsL relA flhD deoC ptsF rbsR23
    W3110F mcrA mcrB IN(rrnD-rrnE)1 λ44
    RG1-BMG1655 csrB::cam (a precise deletion of csrB)22
    MM5057Δ motB urvC-279::Tn10Mike Manson
    MHR 204MC4100 csgA2::Tn10523
    RH106MC4100 rpoS::Tn10R. Hengge-Aronis
    SG20043MC4100 cpsE::Tn10Valerie Stout
    TK821MC4100 ompR331::Tn1044
    TR1-5 or TRacsrA::kanR40
    glgAbglgA::kanRThis study
    DJ1MG1655 csgA2::Tn105This study
    DJ2TRMG1655 csgA2::Tn105This study
    DJ3MG1655 cpsE::Tn10This study
    DJ4TRMG cpsE::Tn10This study
    DJ6TRAAECO72This study
    DJ7AAECO72 csgA2::Tn105This study
    DJ8TRAAECO72 csgA2::Tn105This study
    DJ9AAECO72 cpsE::Tn10This study
    DJ10TRAAECO72 cpsE::Tn10This study
    DJ11MG1655 csgA2::Tn105 cpsE::Tn10This study
    DJ12TR MG1655 csgA2::Tn105 cpsE::Tn10This study
    DJ13AAECO72 csgA2::Tn105 cpsE::Tn10This study
    DJ14TRAAECO72 csgA2::Tn105 cpsE::Tn10This study
    DJ21MG1655 ΔmotB uvrC-279::Tn10This study
    DJ22AAECO72 ΔmotB uvrC-279::Tn10This study
    DJ24TR ΔmotB uvrC-279::Tn10This study
    DJ25TRAAECO72 ΔmotB uvrC 279::Tn10This study
    DJ30MG1655 rpoS::Tn10This study
    DJ31TR MG1655 rpoS::Tn10This study
    DJ32MG1655 rpoS::Tn10 csgA2::Tn105This study
    DJ33TR MG1655 rpoS::Tn10 csgA2::Tn105This study
    DJ34AAECO72 rpoS::Tn10This study
    DJ35TR AAECO72 rpoS::Tn10This study
    DJ36AAECO72 csgA2::Tn105 rpoS::Tn10This study
    DJ37TRAAECO72 csgA2::Tn105 rpoS::Tn10This study
    DJ40MG1655 ompR333::Tn10This study
    DJ41TR MG1655 ompR333::Tn10This study
    DJ42MG1655 ompR333::Tn10 csgA2::Tn105This study
    DJ43TR MG1655 ompR333::Tn10 csgA2::Tn105This study
    DJ44AAECO72 ompR333::Tn10This study
    DJ45TR AAECO72 ompR333::Tn10This study
    DJ46AAECO72 ompR333::Tn10 csgA2::Tn105This study
    DJ47TR AAECO72 ompR333::Tn10 csgA2::Tn105This study
Clinical strains
    C. freundii P524
    E. coli P1824
    E. coli O157:H7 EF30226
    S. enterica serovar Typhimurium ATCC 140284
Bacteriophages
    P1virStrictly lytic P1; forms clear plaquesCarol Gross
    λInCh1For chromosomal integration of genes11
Plasmids
    pUC19Cloning vector, Ampr44
    pBR322Cloning vector, Ampr Tetr44
    pCSR10E. coli csrA gene in pUC19, Ampr40
    pSTCSR5S. enterica serovar Typhimurium csrA in pUC19, GenBank accession no. 276860, AmprThis study
    pCSRH6-19Produces recombinant CsrA under IPTG induction, Ampr27
    pCSRB-SFMinimal csrB in pUC18, Ampr27
    pOP12Contains asd-glgCAP′ in pBR322 Tetr25
    pOP245glgA in pBR322, Tetr25
    pJF02glgP in pUC19, Ampr51
    pMLB1034For construction of ′lacZ translational fusions, Ampr41
    pTR151P1PstI subclone from pTR151, csrA::kanR allele in pUC19, Ampr40
  • a Indicates that the wild-type csrA allele has been replaced by csrA::kanR.

  • b Strains names with the suffix glgA contained a glgA::kanR insertion.