TABLE 1.

PCR primers utilized for the amplification of apsA gene fragments

PrimerSequence (5′→3′)ePrimer binding siteaReference
APS-FWTGG CAG ATM ATG ATY MAC GG481–5007b
APS-RVGGG CCG TAA CCG TCC TTG AA847–8667
APS-uni-FTGG CAG ATV ATG ATY MAC GG481–500This studyc
APS7-FGGG YCT KTC CGC YAT CAA YAC206–236This study
APS7a-FGGG YCT SAG CGC YAT CAA Y206–234This studyd
APS7b-FGG YCT STC CGC YAT CAA Y205–234This studyd
APS8-RGCA CAT GTC GAG GAA GTC TTC1139–1159This study
  • a Positions of the Desulfovibrio-vulgaris apsA open reading frame.

  • b Published primer sequence contains an additional G at the 3′ end, which was a typing error (B. Deplancke, personal communication).

  • c Primer is a modification of primer APS-FW.

  • d Primer is a modification of primer APS7-F.

  • e Degenerate positions are in boldface.