PCR primers utilized for the amplification of apsA gene fragments
| Primer | Sequence (5′→3′)e | Primer binding sitea | Reference |
|---|---|---|---|
| APS-FW | TGG CAG ATM ATG ATY MAC GG | 481–500 | 7b |
| APS-RV | GGG CCG TAA CCG TCC TTG AA | 847–866 | 7 |
| APS-uni-F | TGG CAG ATV ATG ATY MAC GG | 481–500 | This studyc |
| APS7-F | GGG YCT KTC CGC YAT CAA YAC | 206–236 | This study |
| APS7a-F | GGG YCT SAG CGC YAT CAA Y | 206–234 | This studyd |
| APS7b-F | GG YCT STC CGC YAT CAA Y | 205–234 | This studyd |
| APS8-R | GCA CAT GTC GAG GAA GTC TTC | 1139–1159 | This study |
↵a Positions of the Desulfovibrio-vulgaris apsA open reading frame.
↵b Published primer sequence contains an additional G at the 3′ end, which was a typing error (B. Deplancke, personal communication).
↵c Primer is a modification of primer APS-FW.
↵d Primer is a modification of primer APS7-F.
↵e Degenerate positions are in boldface.