TABLE 2.

CAT activities of the S. mutans UA159 fructose-specific EII mutantsa

GenotypeCAT activity (nmol min−1 mg of protein−1)b of strain grown on TV medium with:
InulinInulin and glucoseGlucose
fruCD+ frul+/PfruA-I18.50 ± 3.250.02 ± 0.01NDc
fruI/PfruA-I35.36 ± 4.526.87 ± 1.414.71 ± 0.68
fruCD/PfruA-I27.41 ± 1.190.06 ± 0.040.03 ± 0.03
fruI fruCD/PfruA-I45.13 ± 2.898.82 ± 1.036.07 ± 0.14
fruCD+ fruI+/DC-I83.78 ± 4.2516.60 ± 3.151.89 ± .54
fruI/DC-I77.63 ± 1.67118.80 ± 5.0252.11 ± 6.57
fruCD/DC-I86.58 ± 2.27116.06 ± 22.0462.44 ± 13.5
fruI fruCD/DC-I102.16 ± 9.86140.82 ± 12.865.17 ± 12.21
  • a S. mutans UA159 and its fructose-specific EII mutants, carrying fruCD, fruI, and fruCD fruI, with either PfruA-′cat (PfruA-I) or PfruAΔCRE-′cat (DC-I, PfruA with CRE-S and its 3′UTR deleted) integrated in the chromosome were grown on TV medium with the indicated sugar(s) as the sole carbohydrate source.

  • b CAT activities were measured as described in the text. The data are means ± standard deviations from three separate experiments.

  • c ND, not detectable.