TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant propertiesaReference or source
Strains
    S. enterica serovar Typhimurium
        χ3306Virulent strain, gyrA53
        CS2022lon::Cm in χ3306This study
        χ3306 phoP::KmphoP::Km in χ330634
        CS2099CS2022 harboring pTKY530This study
        CS2110hilA::Tn5lacZY in χ3306This study
        CS2114invF::Tn5lacZY in χ3306This study
        CS2116sipA::Tn5lacZY in χ3306This study
        CS2118sipC::Tn5lacZY in χ3306This study
        CS2130hilA::Tn5lacZY in CS2022This study
        CS2134sipA::Tn5lacZY in CS2022This study
        CS2136sipC::Tn5lacZY in CS2022This study
        CS2138invF::Tn5lacZY in CS2022This study
        CS2152hilA::Tn5lacZY in χ3306 phoP::KmThis study
        CS2156invF::Tn5lacZY in χ3306 phoP::KmThis study
        CS2158sipA::Tn5lacZY in χ3306 phoP::KmThis study
        CS2160sipC::Tn5lacZY in χ3306 phoP::KmThis study
        CS2239CS2022 harboring pMW119This study
        CS2347χ3306 harboring pMW119This study
    E. coli
        SM10(λpir)thri thr leu tonA lacY supE recA::RP4-2Tc::MuKm λpirOur collection
        DH5αF recA endA gyrA thi hsdR supE relA Δ(lacZYA-argF) deoR φ80laclacZ)M15Our collection
Plasmids
    pTKY229Carrying oriγ of R6K and oriT of RP4Our collection
    pNK2884Carrying a Cm gene53
    pT7Blue-2Cloning vectorOur collection
    pMW119Low-copy-number plasmidOur collection
    pTKY501pT7Blue-2 with a 0.5-kb fragment containing a part of lon amplified by PCRThis study
    pTKY503pTKY501 carrying lon::CmThis study
    pTKY513pTKY229 with a 1.6-kb PstI-HindIII fragment carrying lon::CmThis study
    pTKY530pMW119 carrying the lon geneThis study
  • a Abbreviations: Cm, chloramphenicol resistance; Km, kanamycin resistance, Tc, tetracycline resistance.