TABLE 3.

Selected mitochondrially targeted genes down-regulated in rox1 null strains under aerobiosis

Functional groupORFGeneProtein or functionRox1 effectAir/N2 effect
PRatioaPRatiob
Cytoplasmic NADH importYNL055CPOR1Major outer mitochondrial membrane voltage-dependent anion channel protein6.9e−31.54.0e−62.3
YIL155CGUT2FAD-dependent glycerol-3-phosphate dehydrogenase3.4e−21.29.5e−54.9
TCA complexesYFL018CLPD1Dihydrolipoamide dehydrogenase, (E3) component of PDH4.8e−21.32.4e−11.4
YNR001CCIT1Citrate synthetase3.3e−41.61.1e−31.8
YLR304CACO1Aconitase1.8e−21.44.5e−32.1
YDL066WIDP1Isocitrate dehydrogenase (NADP+)4.1e−31.32.4e−21.4
YIL125WKGD1α-Ketoglutarate dehydrogenase2.2e−31.65.3e−41.9
YKL148CSDH1Succinate dehydrogenase flavoprotein subunit5.4e−31.57.2e−42.2
YDR178WSDH4Succinate dehydrogenase membrane anchor subunit1.5e−21.41.2e−32.2
YPL262WFUM1Fumarate hydratase1.4e−31.51.8e−63.2
Assembly facilitators and components of respiratory complexes III, IV, and VYGR174CCBP4Ubiquinol-cytochrome c reductase assembly factor4.3e−41.58.4e−31.7
YML129CCOX14Cytochrome c oxidase assembly protein6.8e−31.43.0e−21.6
YLR038CCOX12Cytochrome c oxidase subunit VIb4.0e−21.38.7e−84.9
YKL087CCYT2Holocytochrome c1 synthase7.5e−31.51.6e−32.1
YER154WOXA1Assembly factor for F1F0-ATP synthase and cytochrome c oxidase2.2e−21.22.5e−31.5
YPL271WATP15Epsilon subunit of F1-ATP synthase2.4e−21.34.5e−21.7
  • a Mean transcript level in wild-type strains divided by that in rox1 strains pooled across genetic backgrounds and media.

  • b Mean transcript level in wild-type strains grown under aerobiosis compared to that grown under anaerobiosis.