TABLE 1.

E. coli strains and plasmids used in this study

Strain or plasmidRelevant genotype or phenotypeSource or reference
Strains
    JM83 ara Δ(lac-proAB) rpsL thi φ80 dlacZΔM15 38
    NF83 recA56 ara Δ(lac-proAB) rpsL thi φ80 dlacZΔM15 29
    BL21(DE3) dcm ompT hsdS gal/λ(DE3) 35
    TN102= W3110 Nalr 11
Plasmids
    pUC119Apr, pMB1 ori, lacZ 38
    pUH23aApr, pMB1 ori, lac promoter, 6-His tagThis study
    pET11aApr, pMB1 ori, T7 promoter 35
    pET28bKmr, pMB1 ori, T7 promoter, 6-His tag 35
    pET11 km-GSTKmr, pMB1 ori, T7 promoter, GST fusion 20
    pKK641A′Kmr, R64drd-11 derivative carrying 18.5-kb rep and pil segment 11
    pKK641A′ pilK1pKK641A′ carrying the pilK1 frameshift mutation 40
    pKK641A′ pilR2pKK641A′ carrying the pilR2 frameshift mutation 40
    pKK641A′ pilT1pKK641A′ carrying the pilT1 frameshift mutation 40
    pKK661Cmr, pHSG576 (pSC101 rep) derivative carrying 35.6-kb tra segment 11
    pKK698a0.8-kb pilM fragment in pUC119 40
    pKK700a2.0-kb pilO fragment in pUC119 40
    pKK701a1.0-kb pilP fragment in pUC119 40
    pEO281.3-kb pilO fragment in pET28bThis study
    pEP280.5-kb pilP fragment in pET28bThis study
    pEM-GST0.5-kb pilM fragment in pET11 km-GSTThis study
    pEP110.5-kb pilP fragment in pET11aThis study
    pUK230.6-kb pilK fragment in pUH23aThis study
    pUR231.1-kb pilR fragment in pUH23aThis study
    pUT230.5-kb pilT fragment in pUH23aThis study