TABLE 3.

Properties of R64 pil gene products

GeneNo. of amino acidsMol massLocationbgsp homologueDescription and reference
Calculated (Da)Found (kDa)aPredictedFound
pilK 19623,18430 [+His]IMIM
pilL 35538,60338IM/OMOMLipoprotein (28)
pilM 14515,71616P/OMIM
pilN 56059,84857M/OMOM gspD Secretin (28)
pilO 43148,16248IMOM
pilP 15016,00320IM
matc12913,64616P/OM
pilQ 51758,28058CC gspE ATPase (29)
pilR 36541,41544 [+His]IMIM gspF Integral membrane protein
pilS 20421,26522IM gspGHIJ Prepilin (39)
mat18118,71519ECPilin (39)
pilT 18620,758IM
mat16118,14024 [+His]PPutative muramidase
pilU 21824,600NTdIMNT gspO Prepilin peptidase (39)
pilVA43045,75844P/OMECAdhesin (10, 39)
  • a [+His], molecular mass was estimated for the protein containing a six-His tag.

  • b C, cytoplasm; IM, inner membrane; OM, outer membrane; P, periplasm; EC, extracellular.

  • c mat, mature form.

  • d NT, not tested.