TABLE 3.

Properties of R64 pil gene products

GeneNo. of amino acidsMol massLocationbgsp homologueDescription and reference
Calculated (Da)Found (kDa)aPredictedFound
pilK19623,18430 [+His]IMIM
pilL35538,60338IM/OMOMLipoprotein (28)
pilM14515,71616P/OMIM
pilN56059,84857M/OMOMgspDSecretin (28)
pilO43148,16248IMOM
pilP15016,00320IM
matc12913,64616P/OM
pilQ51758,28058CCgspEATPase (29)
pilR36541,41544 [+His]IMIMgspFIntegral membrane protein
pilS20421,26522IMgspGHIJPrepilin (39)
mat18118,71519ECPilin (39)
pilT18620,758IM
mat16118,14024 [+His]PPutative muramidase
pilU21824,600NTdIMNTgspOPrepilin peptidase (39)
pilVA43045,75844P/OMECAdhesin (10, 39)
  • a [+His], molecular mass was estimated for the protein containing a six-His tag.

  • b C, cytoplasm; IM, inner membrane; OM, outer membrane; P, periplasm; EC, extracellular.

  • c mat, mature form.

  • d NT, not tested.