TABLE 2.

Effect of mutation of polar and lateral flagellar genes of A. caviae Sch3N on lateral flagellum expression and swarming motilitya

StrainMutationPolar flagellabLateral flagellabSwimming phenotypecSwarming phenotyped
Wild type
    A. caviae Sch3++++++
Polar flagellar mutants
    AAR269 flaA::Kmr++++
    AAR27 flaB::Kmr+++++
    AAR31 flaA::CmrflaB::Kmr
    AAR150 flaG::Kmr++++++
    AAR59 flaH::Kmr
    AAR8 flaJ::Kmr
Lateral flagellar mutants
    AAR58 lafA1::Kmr++++
    AAR5 lafA2::Kmr++++
    AAR9 lafB::Kmr++
    AAR20 fliU::Kmr++++
    AAR6 lafA1::CmrlafA2::Kmr++
  • a Strains were tested in duplicate in at least two separate experiments, and the wild-type strain, A. caviae Sch3, was included in all experiments.

  • b Flagellum expression was determined by TEM and Western blotting (10).

  • c Swimming was determined as migration from the source of inoculation in LB broth containing 0.3% agar (Oxoid) after 16 to 24 h at 30°C. Symbols: ++, spreading zone ≥7 cm from central point of inoculation; +, spreading zone ≥2 cm but <7 cm from the inoculation point; −, growth at the inoculum site but no movement.

  • d Swarming was determined as surface migration on Eiken agar swarm plates (0.5% agar in Difco nutrient broth) after 16 to 24 h at 30°C. Symbols: ++, surface motility zone ≥8 cm; +, surface motility zone ≥3 cm but <8 cm; or −, growth at the inoculum site but no surface movement.