TABLE 2.

Oligonucleotides used in this study

PrimerSequence (5′→3′)a
ltbY18AsenseCACAACACACAAATAGCCACGATAAATGACAAG
ltbY18AantisenseCTTGTCATTTATCGTGGCTATTTGTGTGTTGTG
ltbQ3LsenseCACACGGAGCTCCTCTGTCTATTACAGAACTATG
ltbQ3LantisenseCATAGTTCTGTAATAGACAGAGGAGCTCCGTGTG
ltbQ3KsenseCACACGGAGCTCCTAAATCTATTACAGAACTATG
ltbQ3KantisenseCATAGTTCTGTAATAGATTTAGGAGCTCCGTGTG
ltbT47AsenseGAGCGGCGCAGCATTTCAGGTCGAAGTCCC
ltbT47AantisenseGGGACTTCGACCTGAAATGCTGCGCCGCTC
ltbA46DsenseCATTACATTTAAGAGCGGCGACACATTTCAGGTCG
ltbA46DantisenseCGACCTGAAATGTGTCGCCGCTCTTAAATGTAATG
eltABforwardCGGGATCCCGCCTCGATGAAAAATATAAC
eltABreverseAACTGCAGAACCAATGCATTGGCTAGTTTCCATACTGATTG
dsbABamHIfwdCGGGATCCCGGAAGAATTTAGCGAGTTC
DsbA-R(XbaI)GCTCTAGAGCTCCCTGATAAAACAGCAA
  • a Mutagenic codons are bold; restriction sites are italic.