TABLE 1.

Bacterial strains, plasmids, and cell lines used in this study

Strain, plasmid, or cell lineGenotype or relevant characteristic(s)aReference or source
E. coli strains
    DH5αrecA1 endA1 gyrA96 thi1 hsdR17 supE44 relA1 lacZΔM15Laboratory stock
    BL21(DE3)F ompT gal dcm lon hsdSB(rB mB) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 Sam7 nin5])Novagen
S. pneumonia strains
    D39Encapsulated, type 23
    R6Nonencapsulated, type 213
    KEH001D39; ΔbgaC::ermB ErrThis study
    KEH002R6; ΔbgaC::ermB ErrThis study
Plasmid pET28a+5.4 kb; KanrNovagen
Cell lines
    A549Lung carcinoma epithelial cell (adherent)ATCC CCL-185
    HEp-2Larynx carcinoma epithelial cellATCC CCL-23
  • a Err, erythromycin resistance.