TABLE 2.

Expression of indA::, indB::, and indC::uidA fusions in wild-type and pecS backgroundsa

Carbon sourceAdditional compoundβ-Glucuronidase sp. act. (U) in fusion
indA::uidAindB::uidAindC::uidA
Wild typepecS backgroundWild typepecS backgroundWild typepecS background
Glycerol2307,500752,00012,100
Galactose1203,100451,30011,200
Glucose803,100351,3001800
PGA1352,4002580011,200
GlycerolPlant extract2507,050501,60011,800
GlycerolCasamino Acids1857,900952,20012,200
GlycerolGln + B62057,800802,00012,000
GlycerolParaquat97012,0002503,60044,500
  • a Cultures were grown in M63 minimal medium in the presence of various carbon sources and under different physiological conditions. Cultures were normally grown at 30°C. Carbon sources were added at 2 g · liter−1 except polygalacturonate (PGA), which was added at 4 g · liter−1. The following other compounds were added at the concentrations indicated: plant extract, 1% (vol/vol); Casamino Acids, 40 μg · ml−1; glutamine (Gln), 40 μg · ml−1, plus pyridoxine (vitamin B6), 200 μg ml−1; paraquat, 4 μM. The results reported are the average of three independent experiments; the standard deviation was less than 15% in each case except for low activities (<10 U), for which the standard deviation was greater (able to reach 40%).