TABLE 2.

Specificity-conferring amino acids of adenylation domains in the polymyxin synthetase

A-domainActive site residue at position: Amino acid specificity
235236239278299301322330331517
PmxA A1DAWIVGAIVKLeu
PmxA A2DFWNIGMVHKThr
PmxA A3DVGEISAIDKDab
PmxA A4DVGEISAIDKDab
PmxB A1DFWNIGMVHKThr
PmxE A1DVGEISSIDKDab
PmxE A2DFWNIGMVHKThr
PmxE A3DVGEISSIDKDab
PmxE A4DVGEISAIDKDab
PmxE A5DVGEISAIDKDab