TABLE 1.

Strains and plasmids used in this study

Species and strain or plasmid (antibiotic)aDescriptionReference
E. faecalis
    OG1SS/pCF10 (Str, Tet) prgB + srtA + srtC + 12
    OG1SS/pCF10 ΔSrtA (Str, Tet) prgB + srtA srtC + This study
    OG1SS/pCF10 ΔSrtC (Str, Tet) prgB + srtA + srtC This study
    OG1RF (Rif, Fus) prgB srtA + srtC 10
    OG1X (Str) prgB srtA + srtC 12
    OG1X ΔSrtA (Str) prgB srtA srtC + This study
    OG1X ΔSrtC (Str) prgB srtA + srtC This study
    OG1X ΔSrtA ΔSrtC (Str) prgB srtA srtC This study
E. coli MC1061 50
Plasmids
    pABG5 (Kan, Cm)Shuttle vector 16
    pJRS233 (Erm)Temperature-sensitive plasmid for generation of deletions 33
    pAL1 (Kan)Derivative of pABG5This study
    pAL1::SrtA (Kan)    lacking Cmr This study
    pAL1::SrtA-HA (Kan)    cassetteThis study
    pAL1::SrtA-HA, native srtA promoter (Kan)This study
    pAL1::SrtC-HA (Kan)This study
    pAL1::SrtC-HA(+/−)tail (Kan)This study
    pAL1::SrtC-HA(−)tail (Kan)This study
    pAL1::SrtC-HAΔtail (Kan)This study
  • a Str, streptomycin; Tet, tetracycline; Kan, kanamycin; Erm, erythromycin; Rif, rifampin; Fus, fusidic acid. See Materials and Methods for antibiotic concentrations.