TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype and/or phenotypeaSource or reference
P. aeruginosa
PAO1Wild-type laboratory strainLab collection
PA14Human clinical isolate; Rifr45
JY2MpilC mutant of PA14; Rifr CbrThis study
JY5MpilA mutant of PA14; Rifr CbrThis study
JY11MybtQ mutant of PA14; Rifr CbrThis study
JY15MuvrD mutant of PA14; Rifr CbrThis study
E. coli
DH5αF endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1 Δ(lacZYA-argF)U169 λ φ80 dlacZΔM15Gibco BRL
SM10thi-I thi leu tonA lacY supE recA::RP4-2-Tc::Mu; KmrLab collection
Plasmids
pUC19Cloning vector; AprLab collection
pEX18Suicide vector for P. aeruginosa; oriT+ sacB+, gene replacement vector with MCS from pUC18; Apr23
pGEM-T EasyPCR cloning vector; AprPromega
pJY1pEX18 derivative carrying RDA product; AprThis study
pJY2pEX18 derivative carrying RDA product; AprThis study
pJY3pEX18 derivative carrying RDA product; AprThis study
pJY4pEX18 derivative carrying RDA product; AprThis study
pJY5pEX18 derivative carrying RDA product; AprThis study
pJY6pEX18 derivative carrying RDA product; AprThis study
pJY8pEX18 derivative carrying RDA product; AprThis study
pJY10pEX18 derivative carrying RDA product; AprThis study
pJY11pEX18 derivative carrying RDA product; AprThis study
pJY12pEX18 derivative carrying RDA product; AprThis study
pJY13pEX18 derivative carrying RDA product; AprThis study
pJY15pEX18 derivative carrying RDA product; AprThis study
pJY16pEX18 derivative carrying RDA product; AprThis study
pJY17pEX18 derivative carrying RDA product; AprThis study
pJY19pEX18 derivative carrying RDA product; AprThis study
pJY20pEX18 derivative carrying RDA product; AprThis study
pJY22pEX18 derivative carrying RDA product; AprThis study
pJY23pEX18 derivative carrying RDA product; AprThis study
pJY24pEX18 derivative carrying RDA product; AprThis study
pJY25pEX18 derivative carrying RDA product; AprThis study
pJYP25pGEM-T Easy with a 3.0-kbp fragment of pilABC gene cluster; AprThis study
pJY2ApGEM-T Easy with a 1,160-bp IPCR product of pilC; AprThis study
pJY5ApGEM-T Easy with a 1,030-bp IPCR product of pilA; AprThis study
pJY15ApGEM-T Easy with a 1,150-bp IPCR product of uvrD; AprThis study
pJY2-1pGEM-T Easy with a 600-bp internal fragment of pilC; AprThis study
pJY5-1pGEM-T Easy with a 500-bp internal fragment of pilA; AprThis study
pJY11-1pGEM-T Easy with a 1.3-kbp internal fragment of ybtQ; AprThis study
pJY15-1pGEM-T Easy with a 1.1-kbp internal fragment of uvrD; AprThis study
pJY2MpEX18 derivative carrying a 600-bp internal fragment of pilC; AprThis study
pJY5MpEX18 derivative carrying a 500-bp internal fragment of pilA; AprThis study
pJY11MpEX18 derivative carrying a 1.3-kbp internal fragment of ybtQ; AprThis study
pJY15MpEX18 derivative carrying a 1.1-kb internal fragment of uvrD; AprThis study
pJYYBT2.1-kb EcoRI fragment containing the intact ybtQ gene cloned into pUC19This study
  • a Apr, ampicillin resistance; Cbr, carbenicillin resistance; Kmr, kanamycin resistance; Rifr, rifampin resistance.