TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or characteristicsSource or reference
B. (cepacia) vietnamiensis
G4Typed as B. vietnamiensis by J. LiPuma (personal communication)15
FMT-05 fabF mutant derived from G4This study
IMT-61 bviI mutant derived from G4This study
RMT-14 bviR mutant derived from G4This study
E. coli
DH5α supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA127
MG4Δ(argF-lac)U169 zah-735::Tn 10 recA56 srl::Tn 1026
S17-1 (λpir) recA thi pro hsdR 32
VJS533 recΔA56 ara Δ(lac-proAB)X111 rpsL (φ80 lacZΔM15)35
XL-1 Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F" proAB lacIqZΔM15 Tn 10 (Tetr)]Stratagene
R. solanacearum
AW1-AI8 sol18::SP2
Plasmids
p395B sol reporter; aidA::lacZ, Tcr2
pBBR1MCS-2Mobilizable broad-host-range cloning vector, Kmr11
pBBR1MCS-5Mobilizable broad-host-range cloning vector, Gmr11
pBCL5-28.5-kb HindIII fragment containing the bviI, bviR, and fabF-like gene from B. vietnamiensis G4 in pUC19This study
pBCSFMA 952-bp Rsr II fragment of the fabF-like gene from pBCL-2 was replaced with the pBBR1MCS-5 Gmr marker; the resulting DNA was digested with PstI, treated with the Klenow fragment, and cloned into the EcoRV site in pSUP102This study
pBCSIM bviI, fabF-like gene PstI fragment from pBCL5-2 treated with Klenow fragment and blunt-end cloned into EcoRV-digested pSUP102; a 570-bp BsiW bviI fragment replaced with a Gmr fragment PCR amplified from pBBR1MCS-5This study
pBCSRM4.4-kb bviR bviI EcoRV fragment from pBCL5-2 cloned into the EcoRV site of pSUP102, 1-kb Gmr marker from pBBR1MCS-5 cloned into unique SfiI siteThis study
pECP61.5 rhl reporter; rhlR rhlA::lacZ Apr24
pHV200I lux reporter; luxR luxI"CDABE Apr22
pHV300I lux reporter; luxR luxI"CDABE Cmr7
pKDT17 las reporter; lasB::lacZ plac-lasR Apr21
pSUP102 E. coli-specific mobilizable vector Cmr Tcr32