Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or characteristicsSource or reference
B. (cepacia) vietnamiensis
G4Typed as B. vietnamiensis by J. LiPuma (personal communication)15
FMT-05 fabF mutant derived from G4This study
IMT-61 bviI mutant derived from G4This study
RMT-14 bviR mutant derived from G4This study
E. coli
DH5α supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA127
MG4Δ(argF-lac)U169 zah-735::Tn 10 recA56 srl::Tn 1026
S17-1 (λpir) recA thi pro hsdR 32
VJS533 recΔA56 ara Δ(lac-proAB)X111 rpsL (φ80 lacZΔM15)35
XL-1 Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[F" proAB lacIqZΔM15 Tn 10 (Tetr)]Stratagene
R. solanacearum
AW1-AI8 sol18::SP2
p395B sol reporter; aidA::lacZ, Tcr2
pBBR1MCS-2Mobilizable broad-host-range cloning vector, Kmr11
pBBR1MCS-5Mobilizable broad-host-range cloning vector, Gmr11
pBCL5-28.5-kb HindIII fragment containing the bviI, bviR, and fabF-like gene from B. vietnamiensis G4 in pUC19This study
pBCSFMA 952-bp Rsr II fragment of the fabF-like gene from pBCL-2 was replaced with the pBBR1MCS-5 Gmr marker; the resulting DNA was digested with PstI, treated with the Klenow fragment, and cloned into the EcoRV site in pSUP102This study
pBCSIM bviI, fabF-like gene PstI fragment from pBCL5-2 treated with Klenow fragment and blunt-end cloned into EcoRV-digested pSUP102; a 570-bp BsiW bviI fragment replaced with a Gmr fragment PCR amplified from pBBR1MCS-5This study
pBCSRM4.4-kb bviR bviI EcoRV fragment from pBCL5-2 cloned into the EcoRV site of pSUP102, 1-kb Gmr marker from pBBR1MCS-5 cloned into unique SfiI siteThis study
pECP61.5 rhl reporter; rhlR rhlA::lacZ Apr24
pHV200I lux reporter; luxR luxI"CDABE Apr22
pHV300I lux reporter; luxR luxI"CDABE Cmr7
pKDT17 las reporter; lasB::lacZ plac-lasR Apr21
pSUP102 E. coli-specific mobilizable vector Cmr Tcr32