TABLE 2.

Identification of selected protein spots that were differentially expressed over the course of biofilm developmenta

Class and functionSpot no.ProteinLocusDevelopment stage of maximum protein concnbMolecular mass (kDa)pINo. of matched peptides
Class I
Carbon catabolism and amino acid metabolism1Arginine deiminaseArcA, PA517112d46.305.5224
8Ornithine carbamoyltransferse, catabolicArcB, PA517212d37.976.1417
15Carbamate kinaseArcC, PA517312d33.085.2513
2Fumarate hydratase C1PA447012d48.75.6515
3Class III aminotransferase, probablePA24136d50.226.0317
4Acylase, probablePA238512d84.046.6427
5Dihydrolipoamide dehydrogenase 3PA4829P50.026.4819
7Glyceraldehyde-3-phosphate dehydrogenasePA30011d/6d50.117.7415
9Ketol-acid reductoisomerasePA469412d36.435.5719
16UTP-glucose-1-phosphate uridyltransferasePA202312d31.245.3314
17Succinate semialdehyde dehydrogenasePA026512d51.625.5918
Cofactor biosynthesis6 l-Ornithine 5-monooxygenase (pyoverdine biosynthesis)PA23866d49.476.0324
15Thioredoxin reductase (pyrimidine biosynthesis)PA261612d33.825.249
20Adenylate kinase (purine biosynthesis)PA368612d23.15.9811
Class II (fatty acid, phospholipid, LPS)23β-Hydroxydecanoyl-ACP dehydrogenaseFabA, PA008512d18.756.413
Class III (membrane proteins, transport)4Probable binding protein component of ABC transporterPA449612d60.087.1113
19Porin E1PA029112d49.678.6723
20Probable binding protein component of ABC transporterPA507612d59.746.857
Class IV (adaptation, protection)11Alkyl hydroxyperoxide reductase subunit CPA013912d20.545.897
12Superoxide dismutasePA446812d22.615.815
10Probable ribosomal protein L25PA46716d21.965.8110
14Secreted hemolysin-coregulated protein HcpPA026312d19.095.4911
Class V (various)13Conserved hypothetical proteinPA042312d20.786.0914
16Hypothetical proteinPA239512d31.075.4410
21, 22Helix-destabilizing protein of bacteriophage Pf1PA0720P/12d15.476.254
  • a The protein spots were identified by MS analysis. The spot numbers correlate with the numbers indicated in Fig. 6. The locus designates the gene number in the genome of Pseudomonas aeruginosa. Matched peptides, number of peptides that matched with molecular masses of theoretically trypsin-digested peptides of P. aeruginosa proteins.

  • b P, planktonic; 1d, 6d, and 12d, 1-, 6-, and 12-day biofilm, respectively.