TABLE 1

Strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
Strains
    P. aeruginosa
        PAKStrain PAK15
        PAK pilAPAK with a deletion of pilA60
        PAK pilUPAK with a deletion of pilUMatthew C. Wolgang
        PAK cyaABPAK with deletions of cyaA and cyaB15
        PAK cyaB-R456LPAK encoding an arginine-to-leucine change in CyaB at amino acid position 456This study
        PAK fimVPAK with a deletion of fimV15
        PAK fimV cyaB-R456LPAK carrying a deletion of fimV and encoding an arginine-to-leucine change in CyaB at amino acid position 456This study
        PAK fimV1194PAK with an FRT insertion at nucleotide position 1194 of fimVThis study
    E. coli
        BL21(DE3)Protein overexpression strainInvitrogen
        B834(DE3)Methionine auxotrophic protein expression strainInvitrogen
        BTH101Bacterial two-hybrid system reporter strainEuromedex
Plasmids
    pRK793IPTG-inducible expression vector encoding TEV protease41
    pBADGrArabinose-inducible protein expression vector61
    pBADGr::fimVArabinose-inducible vector encoding full-length FimVThis study
    pBADGr::fimV689Arabinose-inducible vector encoding FimV residues 1 to 689This study
    pET151/D-TOPO::fimV862–919IPTG-inducible protein overexpression vector encoding FimV residues 862 to 919This study
    pKNT25IPTG-inducible expression vector encoding T25 fragment of B. pertussis CyaBEuromedex
    pKNT25::fimV488–618IPTG-inducible expression vector encoding T25 fragment of B. pertussis CyaB fused to the C terminus of FimV TPR1+2This study
    pKNT25::fimV811–919IPTG-inducible expression vector encoding T25 fragment of B. pertussis CyaB fused to the C terminus of FimV TPR3This study
    pKNT25::fimLIPTG-inducible expression vector encoding T25 fragment of B. pertussis CyaB fused to the C terminus of FimLThis study
    pKNT25::pilGIPTG-inducible expression vector encoding T25 fragment of B. pertussis CyaB fused to the C terminus of PilGThis study
    pUT18IPTG-inducible expression vector encoding T18 fragment of B. pertussis CyaBEuromedex
    pUT18::fimV488–618IPTG-inducible expression vector encoding T18 fragment of B. pertussis CyaB fused to the C terminus of FimV TPR1+2This study
    pUT18::fimV811–919IPTG-inducible expression vector encoding T18 fragment of B. pertussis CyaB fused to the C terminus of FimV TPR3This study
    pUT18::fimLIPTG-inducible expression vector encoding T18 fragment of B. pertussis CyaB fused to the C terminus of FimLThis study