TABLE 2

Oligonucleotides used in this study

PrimerSequence (5′–3′)
cyaB-R456L F1GCGGGTACCTTCACCGCACTGGTCATCG
cyaB-R456L R1GCGTCTAGAAAGTCAACGGAGTCGTCACC
cyaB-R456L SDM FTGCAACAGGCCGCCGAGCTCCTGCGCGACAAGGTC
cyaB-R456L SDM RGACCTTGTCGCGCAGGAGCTCGGCGGCCTGTTGCA
fimV862–919 FCACCGATGACTTCGACTTCCTCTCCGGTGC
fimV862–919 RTCAGGCCAGGCGCTCCAGCAACTC
FimV FTTAGTACTAAGGGATTACACTATGGTTCGGCT
FimV RCAGTTCTAGATCAATGGTGATGGTGATGATGGGCCAGGCGCTCCAGCAACTC
FimV689 FTAAGAATTCCAAGGGATTACACTATGGTTCGGCTT
FimV689 RAATAAGCTTTCAGAGGTCGGCCTGCACGTCG
B2H FimV TPR1FGCGTCTAGAGATGAATGCGCAGAAAGAGAAGG
B2H FimV TPR1RGCGGGTACCGCCTTGAGCTGCTCGAC
B2H FimV TPR3FGCGTCTAGAGATGGAGAAGGGCGAGGACAG
B2H FimV TPR3RGCGGGTACCGCGGCCAGGCGCTCCAG
B2H FimL-FGCG TCT AGA GAT GGT CAC AGG AGC CAC GTC CC
B2H FimL-RGCG GGT ACC GCG GCG GCC ACC GG
B2H PilG-FGCG TCT AGA AAT GGA ACA GCA ATC CGA CGG
B2H PilG-RTAA GGT ACC CGG GAA ACG GCG TCC ACC