TABLE 1.

Bacterial strains and plasmids

Strain(s) or plasmidRelevant genotype or phenotypeReference
P. aeruginosa strains
    PAO1Wild type
    MJK8Biofilm-derived rugose strain (isogenic to PAO1)21
    MJK8ΔcupA3cupA3 polar mutant of MJK8This study
    MJK8ΔcupA-FLPcupA3 nonpolar mutant of MJK8This study
    MJK8ΔpslpelpslBCD pelA mutant of MJK8This study
    MJK8Δ1169PA1169 mutant of MJK8This study
    CF37wt to CF39wtClinical longitudinal isolates with wild-type phenotype6, 21
    CF38s and CF39sClinical longitudinal isolates with rugose phenotype6, 21
    CF39sΔpslBCDpslBCD mutant of CF39sThis study
    CF39sΔpelApelA mutant of CF39sThis study
    CF39sΔpslBCDΔpelApslBCD pelA mutant of CF39sThis study
    WF801PAO1ΔpslBCD expressing PBAD-psl1
    WF800pslBCD mutant of PAO1 expressing PBAD-psl23
Plasmids
    pFLP2Source of FLP recombinase, Ampr18
    pEX18.ApSuicide cloning vector, Ampr18
    pSP858Source of gentamicin resistance cassette, Ampr Gentr18
    pSP5WspF expresson vector, AmprThis study
    pSP6PA2133 expression, AmprThis study
    pUCP18E. coli-P. aeruginosa expression vector, Ampr34
    pMPSL-KO1pslBCD allelic replacement vector21
    pMPELApelA allelic replacement vectorThis study
    pMCUPA3cupA3 allelic replacement vectorThis study
    pM1169PA1169 allelic replacement vectorThis study