TABLE 1.

E. coli K-12 strains and plasmids used in this study

Strain or plasmidRelevant propertiesSource or reference(s)
Strains
    FS1576 supE44 hsdR thi-1 thr-1 leuB6 lacY1 tonA21 recD1009 13, 17
    JC10240Hfr thi-300 recA56 srl::Tn10 relA1 ilv-318 spoT1 thi rpsE2300 1
    DLP79-36HfrC pps man lppThis study
    CDE4DLP79-36 ΔlolC-lolD-lolE::kan lpp recA56/pMAN1015This study
Plasmids
    pJY310 cat; lolC-lolD-lolE 21
    pMAN997 bla; temperature-sensitive replicon, multicloning sites 19
    pMAN885 cat; multicloning sites 23
    pMAN885EH cat; multicloning sites 23
    pMAN1015 bla; lolC-lolD-lolE temperature-sensitive repliconThis study
    pNASCD cat; arabinose promoter-controlled lolC-lolDThis study
    pNASDE cat; arabinose promoter-controlled lolD-lolEThis study
    pNASCDE cat; arabinose promoter-controlled lolC-lolD-lolEThis study
    pKM601 cat; arabinose promoter-controlled gene for L10PSRThis study
    pKT100 spc; tacPO lppSR This study