TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotypeaSource or reference
Strains
    Y. enterocolitica
        JB580vSerogroup O:8, Nalr ΔyenR (r m+)26
        GY460ΔflhDC56
        GY563flgA::TnMod-RKm"-b
        GY567flgB::TnMod-RKm"-b
        GY648flhB::TnMod-RKm"-b
        GY667fliE::TnMod-RKm"-b
        GY751motA::mTn5Km2-lacZYA-c
        GY824fleB::mTn5Km2-lacZYA-c
        YVM139flgM::Kan29
        VK1fliA::Str29
        YEDS10yplA-lacZYA yplA+46
        YVM356yscR::mTn5Km28
        YVM351yscU::mTn5Km28
        GY4555yscK::TnMod-lacZYA-RKm"-c
        GY4412ysaI::TnMod-RKm", pGY100This study
        GY4418ysaV::TnMod-RKm", pGY100This study
        GY4413ysaW::TnMod-RKm", pGY100This study
        GY4415ysaT::TnMod-RKm", pGY100This study
        GY4414orf1-1::TnMod-RKm", pGY100This study
        GY4416orf1-2::TnMod-RKm", pGY100This study
        GY4499ysaT::TnMod-RKm"This study
        GY4482ΔflhDC, ysaT::TnMod-RKm"This study
        GY4478pYV8081This study
        GY4479ΔflhDC, pYV8081This study
        GY4481ysaT::TnMod-RKm", pYV8081This study
        GY4492ΔflhDC ysaT::TnMod-RKm", pYV8081This study
    E. coli S17-1 λpirrecA thi pro hsdRM+ RP4::2-Tc::Mu::Km Tn7 λpir37
Plasmids
    pGY10flhDC locus in pTM10056
    pGY22ΔflhDC allele in the suicide vector pEP185.256
    pGY100Pcat-yplAB derivative of pTM100This study
    pTM100mob+, derivative of pACYC184, Cmr Tetr33
    pTnMod-RKm"TnMod-RKm" in a delivery vector10
  • a Cmr, chloramphenicol resistance; Tetr, tetracycline resistance.

  • b S. Petersen and G. M. Young, unpublished data.

  • c K. Venecia and G. M. Young, unpublished data.