Description of genes in the regions flanking the hrp gene cluster of X. campestris pv. vesicatoria

GeneClosest protein homolog (organism; accession no.)aIdentity/similarityb (%)Comments
Downstream of hrpA
    ORF1340 aaf
    hpaHHpa2 (X. oryzae pv. oryzae; AAF61278)96/98Imperfect PIP box; hgi8Ig; 157 aa
    xopAHpa1 (X. oryzae pv. oryzae; AAC95121)46/59PIP box; hgi27g; 50% G+C content; 117 aa
    ORF4Putative transposase (Y. pestis; CAB54971)50/78Conserved frameshifting site (CAAAAAAA)
    xopDPsvA (P. syringae pv. eriobotryae; BAA87062)65/74chrp box; 54% G+C content; 612 aa; NLSh
    ORF6Putative transposase (X. campestris pv. mangiferaeindicae; AF249895)74/78Frameshift; part of ISXc 7
Downstream of hrpF
    hpaFHpaF (X. oryzae pv. oryzae; AB045312)81/87dPIP box; hgi203g; 197 aa; NLS
    hpaGHpaF (X. oryzae pv. oryzae; AB045312)87/93e432 aa; LRRs
  • a Closest homologous protein using the BlastX algorithm (37) in nonredundant databases.

  • b Identities and similarities between the respective proteins were determined by using the Blast 2 Sequences algorithm (40).

  • c Refers to the last 300 amino acids of XopD and PsvA.

  • d Refers to the first 180 amino acids of HpaF from X. oryzae pv. oryzae.

  • e Refers to the last 430 amino acids of HpaF from X. oryzae pv. oryzae.

  • f aa, amino acids; refers to the predicted size of the encoded protein.

  • g hrpG-induced cDNA fragment; gene identified by cDNA-AFLP (34).

  • h Putative NLS.