TABLE 1.

Bacterial strains

Species and strainsGenotype
E. coli
    BE11(E. coli ER2267) Fe14 (MrcA) endA1 supE44 thi-1 relA1? rfbD1? spoT1? Δ(mrcC-mrr) 114::IS10 Δ(argF-lac)U169 recA1/FproA+B+laclq Δ(lacZ)M15 zzf::mini-Tn10 (Kanr)/pMGS2
    BE229(E. coli ER1992) Fλ Δ(argF-lac)U169 supE44 e14dind1::MudI1473 (KanrlacZ+) rtbD1? relA1? endA1 spoT1? thi-1 Δ(mrcL-mrr)114::IS10/pGH48 (His6-PduA Apr)
    BE230BL21(DE3)/pGH48(His6-PduA Apr) and pSJS1240 (ileX argU Spr)
    BE231BL21(DE3) RIL/pGH107(T7 expression vector with pduJ insert, Kanr)
    BL21(DE3)(E. coli B) FompT hsdS(rB mB) dcm+ Tetrgal λ (DE3) endA
    BL21(DE3) RIL(E. coli B) FompT hsdS(rB mB) dcm+ Tetrgal λ (DE3) endA Hte (argU ileY leuW Camr)
    RT1679DH5α/pVJ70 (contains pduF"ABCDE ORF1" Apr)
S. enterica serovar Typhimurium LT2Wild-type
    BE33 pduA672:: TPOP1
    BE39 pduA673 TPOP1::fragment (a short nonpolar insertion resulting from the spontaneous deletion of the majority of the pduA672::TPOP1 element)
    BE103TR6579/pKD46a
    BE182ΔpduA652 (precise nonpolar deletion of the pduA gene)b
    BE228ΔpduA652/pTA749 (placIqPO-BglII with pduA insert, Apr)
    BE232 metE205 ara-9 Δ299/placIqPO-BglII (no insert, Apr) (in Δ299 the entire cob and pdu operons are deleted)
    BE233 metE205 ara-9 Δ299/pTA749 (pduA under plac control, Apr) (in Δ299 background the entire cob and pdu operons are deleted)
    BE235 pdu12::MudJ
    TR6579 metA22 metE551 trpD2 ivl-452 hsdLT6 hsdSA29 HsdBstrA120 GalE Leu Pro
  • a See reference 8.

  • b See Materials and Methods.