TABLE 4.

Hemolytic activities of culture supernatants and purified protein

Strain or purified proteinHemolytic activity (%)a
Strains
    lmo0964::Himar1 strain40.3 ± 10.6**
    lmo1268::Himar1 strain59.7 ± 17.1*
    lmo1401::Himar1 strain69.9 ± 3.9**
    lmo1575::Himar1 strain29.6 ± 2.6***
    lmo1821::Himar1 strain101.5 ± 14.3
ΔprsA2 mutant58.0 ± 13.3*
ΔprsA2 mutant + construct 288.7 ± 10.8
Purified protein prsA2::Himar1 LLOHis667.2 ± 6.1**
  • a Mean percentages of the hemolytic activities of either culture supernatants or purified protein ± standard deviations, relative to WT activity, from at least four experiments. Statistical analysis was performed as described for Table 3. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.