TABLE 1.

Oligonucleotide primers, plasmids, and strains used in this study

Primer, plasmid, or strainSequence (5′→3′) or descriptionaRestriction enzyme(s)Reference and/or source(s)
Primers
    112AAAgtcgacTAACAGGTTGGCTGATAAGTCCCSalI
    24AAACTGCAGGTACCCGGGTTCCAGTTTGTAATACGACTCACSmaI, KpnI, PstI
    29AAACTGCAGAAAACTAGTCTCGAGTGGGGTACGCGTAATACGACTCPstI, SpeI, XhoI
    26AAAAAGCTTGGATCCTAACAGGTTGGCTGATAAGTCCCBamHI, HindIII
    30AAACTGCAGCTAAAGTTATGGAAATAAGACTTAGPstI
    31AAAACTAGTATTCAAATTTATCCTTATTGTACAAASpeI
    113AAAGGATCCAATTTTGCAAAAAGTTGTTGACTTTATCTACAAGGTGTGGCATAATGTGTGTCGGCCGATAAAGCAAGCATATAATATTGCGTTTCATCBamHI, EagI
    114AAAAAGCTTGTCGACTTATTCAACATAGTTCCCTTCAAGASalI, HindIII
    134AAACGGCCGATGGAAAAAAAGGAATTTCGTGTTTTGEagI
    229AAAGTCGACTGGAACCGGAGAAGAAACGCSalI
    230GTGTTTTTTAGTGCTTATTTAGATCCAAGAATTAATTTCTTCTTCAT
    231ATGAAGAAGAAATTAATTCTTGGATCTAAATAAGCACTAAAAAACAC
    232AAACTGCAGGAAACAGTAGACCTATTCTTGCPstI
    241AACGGCCGTTTCACACCAATCGGACATTCCEagI
    242AACGGCCGACCATAAGAATATCATTAAATTTCTTTCEagI
    243AACGGCCGATGAAGAAGAAATTAATTCTTGGACEagI
    249AAAGTCGACTAAAGTACTAAAACATACAAAACCGSalI
    MSPY5ACTACGCACCGGACGAGACGTAGCGTC 79
    MSPY3p-CGGACGCTACGTCCGTGTTGTCGGTCCTG 79
Plasmids
    pBADC9 39
    phiMycoMarT7 65
    pUC19Invitrogen
    pLTV3 4
    pKSV7 75
    pPL2 42
    pJZ025This study
    pJZ026This study
    pJZ029This study
    pJZ032This study
    pJZ037This study
    pJZ039This study
    pJZ064This study
    pJZ065This study
    pJZ066This study
L. monocytogenes strains
    10403SWT 3
    DP-L5539 Himar1 transposon generated library, strain 10403SThis study
    DP-L5558Transposon insertion into lmo0964This study
    DP-L5566Transposon insertion into lmo1268This study
    DP-L5570Transposon insertion into lmo1401This study
    DP-L5575Transposon insertion into lmo1609This study
    DP-L5577Transposon insertion into lmo1695This study
    DP-L5580Transposon insertion into lmo1821This study
    DP-L5596Transposon insertion into lmo2219This study
    DP-L5600Transposon insertion into lmo2460This study
    DP-L560110403S Δ prsA(29-291)This study
    DP-L560210403S Δ prsA(29-291) tRNAArg::pPL2 with construct 1This study
    DP-L560310403S Δ prsA(29-291) tRNAArg::pPL2 with construct 2This study
    DP-L560410403S Δ prsA(29-291) tRNAArg::pPL2 with construct 3This study
    DP-L560510403S Δ prsA(29-291) tRNAArg::pPL2This study
    DP-L216110403S Δhly 33
    DP-L560610403S Δhly prsA2::Himar1 This study
    DP-L405710403S LLOS44A 22
    DP-L560710403S LLOS44A prsA2::Himar1 This study
    DP-L436110403S LLOFLAG This study
    DP-L560810403S LLOFLAG prsA2::Himar1 This study
    DP-L861SLCC-5764 44
    DP-L5609SLCC-5764 prsA2::Himar1 This study
    DP-L193510403S ΔplcB 74
    DP-L465010403S ΔflaA 85
    DP-L431710403S ΔprfA H. Shen and J. F. Miller; 10
    DP-L348110403S LLOHis6 20
    DP-L563310403S LLOHis6 prsA2::Himar1 This study
  • a Underlining indicates restriction enzyme sites. Δ prsA(29-291), the deletion in prsA resulting in the removal of amino acids 29 to 291; p-, 5′-phosphate.