TABLE 1.

C. perfringens strains and plasmids used in this study

Strain or plasmidDescriptionaSource
C. perfringens strains
    Strain 13Wild-type strain (type A)
    TS133Strain 13 virR::Tetr
    TS230Strain 13 ΔagrBD::EmrThis study
    TS231Strain 13 ΔagrBDvirR::Tetr EmrThis study
Plasmids
    pJIR418E. coli -C. perfringens shuttle vector, Cmr Emr; pJIR418 Ω (PstI 4.3-kb strain 13 genomic library)
    pTS405virR+ virS+ complementation vector; CmrT. Shimizu, unpublished data
    pTS1301pJIR418 Ω(Δpromoter CPE1562-CPE1561-agrD-CPE1560)This study
    pTS1302pJIR418 Ω(Δpromoter agrD-CPE1560)This study
    pTS1303pJIR418 Ω(CPE1563-CPE1562-CPE1561-agrD -CPE1560) including promoterThis study
    pTS1304pJIR418 Ω(CPE1563-CPE1562-CPE1561-agrD) including promoterThis study
    pTS1305pJIR418 Ω(Δpromoter CPE1562-CPE1561-agrD)This study
    pTS1306pJIR418 Ω(Δpromoter CPE1561-agrD)This study
    pTS1307pJIR418 Ω(Δpromoter agrD)This study
    pTS1308pJIR418 Ω(CPE1562-CPE1561-agrD)This study
    pTS1309pJIR418 Ω(CPE1561-agrD)This study
    pTS1310pJIR418 Ω(agrD)This study
    pTS1311pJIR418 Ω(CPE1563-agrD)This study
    pTS1312pJIR418 Ω(CPE1563-CPE1562-agrD)This study
    pTS1313pJIR418 Ω(CPE1562-agrD)This study
    pTS1314pJIR418 Ω(CPE1562-CPE1561-agrD)This study
  • a Cmr, chloramphenicol resistance; Emr, erythromycin resistance; Tetr, tetracycline resistance.