TABLE 2.

Unique transposon insertions producing a hypohemolytic phenotype, including those with a precisely mapped site

StrainTransposon insertion(s)Gene nameaAnnotationaBlood plate phenotypebRelative plaque size range (%)c
DP-L5540lmo0027Similar to PTS system, beta-glucoside-specific enzyme IIABC-80-100
DP-L5541lmo0031LacI family transcription regulator-ND
DP-L5542lmo0134, lmo0135-80-100
DP-L5543lmo0175, lmo0176-80-100
DP-L5544lmo0201, lmo0202----80-100
DP-L5545lmo0202hlyListeriolysin O----ND
DP-L5547lmo0223cysKSimilar to cysteine synthase-80-100
DP-L5548lmo0234Similar to B. subtilis YacL-80-100
DP-L5550lmo0279Similar to anaerobic ribonucleoside triphosphate reductase-80-100
DP-L5551lmo0352Similar to regulatory proteins (DeoR family)--80-100
DP-L5552lmo0669Similar to oxidoreductase-80-100
DP-L5553lmo0734Similar to transcriptional regulator (LacI family)-ND
DP-L5554lmo0785Similar to transcriptional regulator (NifA/NtrC family)-80-100
DP-L5555lmo0813Similar to fructokinases-80-100
DP-L5556lmo0821---80-100
DP-L5557lmo0833Similar to transcriptional regulator-80-100
DP-L5558lmo0964Similar to B. subtilis YjbH----See Table 3
DP-L5559lmo0964, lmo0965-80-100
DP-L5561lmo1017Similar to phosphotransferase system glucose-specific enzyme IIA-80-100
DP-L5562lmo1079Similar to B. subtilis YfhO-80-100
DP-L5563lmo1254Similar to alpha,alpha-phosphotrehalase--80-100
DP-L5564lmo1255Similar to PTS system, trehalose-specific enzyme IIBC-80-100
DP-L5566lmo1268clpXATP-dependent protease, ATP-binding subunit-See Table 3
DP-L5568lmo1329ribCSimilar to riboflavin kinase and FAD synthase-80-100
DP-L5569lmo1354Similar to aminopeptidase P-ND
DP-L5570lmo1401--See Table 3
DP-L5571lmo1475hrcAHeat-inducible transcription repressor-80-100
DP-L5572lmo1514, lmo1515-80-100
DP-L5573lmo1515-80-100
DP-L5574lmo1575--See Table 3
DP-L5575lmo1609Similar to thioredoxin-80-100
DP-L5576lmo1616-80-100
DP-L5577lmo1695mprF-60-80
DP-L5578lmo1772purCPhosphoribosylaminoimidazole-succinocarboxamide synthase-80-100
DP-L5579lmo1814--80-100
DP-L5580lmo1821prpCSimilar to putative phosphoprotein phosphatase--See Table 3
DP-L5581lmo1829Similar to fibronectin binding proteins-80-100
DP-L5582lmo1878Manganese transport transcriptional regulator-80-100
DP-L5583lmo1922-80-100
DP-L5584lmo1952, lmo1953--80-100
DP-L5586lmo1954drmSimilar to phosphopentomutase--80-100
DP-L5587lmo1974Similar to transcription regulators (GntR family)-80-100
DP-L5590lmo2055-ND
DP-L5591lmo2072, lmo2073-80-100
DP-L5592lmo2103, lmo2104--80-100
DP-L5593lmo2110Similar to mannnose-6 phosphate isomerase-80-100
DP-L5594lmo2203Similar to N-acetylmuramoyl-l-alanine amidase and to internalin B-80-100
DP-L5595lmo2217-ND
DP-L5596lmo2219prsA2Similar to posttranslocation molecular chaperone--See Table 3
DP-L5597lmo2230Similar to arsenate reductase--80-100
DP-L5598lmo2361-80-100
DP-L5599lmo2376Similar to peptidyl-prolyl cis/trans isomerase--ND
DP-L5636lmo2460Similar to B. subtilis CggR hypothetical transcriptional regulator-60-80
DP-L5637lmo2460, lmo2461-80-100
DP-L5638lmo2461sigLRNA polymerase factor sigma-54-80-100
DP-L5639lmo2758guaBSimilar to inosine-monophosphate dehydrogenase-80-100
DP-L5604lmo2770Bifunctional glutamate-cysteine ligase/glutathione synthetase-ND
DP-L5546dlmo0202, lmo0203-ND
DP-L5565dlmo1255, lmo1256---ND
DP-L5567dlmo1295, lmo1296-ND
DP-L5585dlmo1953pnpSimilar to purine-nucleoside phosphorylase--ND
DP-L5588dlmo2016cspBSimilar to major cold shock protein---80-100
DP-L5589dlmo2020divIVASimilar to cell division initiation protein-ND
  • a As identified by NCBI annotation of the L. monocytogenes EGD-e genome. PTS, phosphotransferase system; FAD, flavin adenine dinucleotide. Blank spaces indicate that no specific annotation has been assigned.

  • b Qualitative hemolytic defect visually determined by comparison to an adjacent WT colony on a blood agar plate as follows: -, 75%; - -, 50%; - - -, 25%; - - - -, 0% of the activity of the WT colony.

  • c Relative to the size of the WT in the L2 fibroblast plaque assay. ND, not done.

  • d The approximate site of the transposon is mapped.