TABLE 1.

Distribution of 14C-labeled carboxy acids in M. extorquens AM1 and mutants incubated with 14C-labeled substrates

Strain or mutantLabeled intermediate(s) detected with the following substrate:
[1-14C]butyrate + methanol[1-14C]acetate + methanol[14C]bicarbonate + propionate[1-14C]bicarbonate + butyrate
AM1β-Hydroxybutyrateβ-Hydroxybutyrate, butyrate, methylsuccinate, isobutyrate, β-hydroxyisobutyrate, methylmalonateMethylmalonate, succinateEthylmalonate, methylsuccinate
ccr β-Hydroxybutyrate, methylsuccinate, methacrylate, β-hydroxyisobutyrateNTaNTNT
meaA β-Hydroxybutyrate, methylsuccinate, β-hydroxyisobutyrate, unknown compoundβ-Hydroxybutyrate, butyrate, methylsuccinate, isobutyrate, β-hydroxyisobutyrateNTEthylmalonate, methylsuccinate
ibd2 β-HydroxybutyrateNTNTNT
pccA pccB None detectedβ-HydroxybutyrateNone detectedNone detected
meaB NTβ-Hydroxybutyrate, butyrate, methylsuccinate, isobutyrate, β-hydroxyisobutyrate, methylmalonateMethylmalonateEthylmalonate, methylsuccinate
mcmA NTNTMethylmalonateEthylmalonate, methylsuccinate
epm NTNTMethylmalonate, succinateNT
  • a NT, not tested.