TABLE 1.

Bacterial strains and plasmids

Strain or plasmidGenotype and/or relevant markersReference or source
E. coli
    SY327Δ(lac-pro) argE(Am) rif malA recA56 λpir35
    S17-1thi pro hsdR hsdM+ recA RP4-2-Tc::Mu-Km::Tn7 λpir59
    XL1-BluerecA1 endA1 gyrA96, thi-1 hsdR17 supE44 relA1 Δ(lac-pro) [F′proAB lacIq lacZΔM15 Tn10(Tetr)]Stratagene
V. anguillarum
    NB10Wild type, serotype O1, clinical isolate from the Gulf of Bothnia40
    SY10Deletion of ToxR residues 559-1257This study
    SY11Deletion of ToxS residues 1436-1948This study
Plasmids
    pBluescriptApr; ColE1 originStratagene
    pBSToxS-263Apr; pBluescript containing 263-bp PCR fragment from toxS (bp 1481-1732)This study
    pBSToxS-1Apr; pBluescript containing a cloned fragment with toxRS genesThis study
    pDM4Cmr; suicide vector with an R6K origin (pir requiring) and sacBR genes from Bacillus subtilis37
    pDMToxR1Cmr; pDM4 derivative containing toxR bp 321-558 fused to bp 1258-1496This study
    pDMToxS2Cmr; pDM4 derivative containing toxS bp 1197-1435 fused to bp 1949-2226This study
    pSup202Cmr Tcr Apr; pBR325 derivative (ColE1 origin, RP4 mob+)59
    pDM8Cmr Tcr; pSup202 derivative containing promoterless lacZ gene used for transcriptional gene fusion studies10
    pDM8-ToxRCmr Tcr; pDM8 derivative containing a toxR::lacZ transcriptional gene fusion (residues 297-536)This study
    pSup202PCmr Tcr; pSup202 derivative (ColE1 origin, RP4 mob+) with a polylinker cloned into PstI within the Apr gene36
    pToxRCmr Tcr; pSup202P derivative containing toxR gene and its promoter (residues 321-1496)This study
    pMMB208Cmr IncQ lacIq Ptac, polylinker from M13mp1938
    pMMBToxRCmr; pMMB208 derivative containing toxR gene and its promoter (residues 321-1496)This study