TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant genotype or descriptionSource or reference
V. cholerae
    O395N1ParentalJ. J. Mekalanos
    VcheA-1O395N1 ΔcheA-1This study
    VcheA-2O395N1 ΔcheA-2This study
    VcheA-3O395N1 ΔcheA-3This study
    VcheA-123O395N1 ΔcheA-1, ΔcheA-2, ΔcheA-3This study
    T:ZO395N1 toxT::lacZ6
    T:ZcheA-1O395N1 toxT::lacZ ΔcheA-1This study
    T:ZcheA-2O395N1 toxT::lacZ ΔcheA-2This study
    T:ZcheA-3O395N1 toxT::lacZ ΔcheA-3This study
    T:ZcheA-123O395N1 toxT::lacZ ΔcheA-1, ΔcheA-2, ΔcheA-3This study
    TCP-2O395 tcpA::TnphoAR. K. Taylor
    TCP-2cheA-2TCP-2 ΔcheA-2This study
    KP2.11O395 ctx::phoAR. K. Taylor
    KP2.11cheA-2KP2.11 ΔcheA-2This study
E. coli
    RP437ParentalJ. S. Parkinson
    EcheA(RP9535) RP437 ΔcheAJ. S. Parkinson
Plasmids
    pBAD-TOPOExpression vectorInvitrogen
    pVcheA-1pBAD-TOPO with V. cholerae cheA-1This study
    pVcheA-2pBAD-TOPO with V. cholerae cheA-2This study
    pVcheA-3pBAD-TOPO with V. cholerae cheA-3This study
    pEcheApBAD-TOPO with E. coli cheAThis study