TABLE 1.

List of strains and plasmids used in this study

Strain or plasmidRelevant phenotype, genotype, or characteristic(s)Source or reference
Strains
    Acinetobacter sp. ADP1Grows on C12-C16 alkanes 23
    A. borkumensis AP1Grows on C8-C16 alkanes 62
    E. coli JM101 endA hsdR, supR, thi-I, Δ(lac-proAB) F′ (traD36, proAB, laclq, lacZM15) 63
    E. coli DH10BCloning strainGibco BRL
    E. coli SF800W3110 polA mutantLaboratory collection
    E. coli GEc137DH-1 thi, fadR 8
    P. aeruginosa PAO1Grows on C12-C24 alkanes 21
    P. fluorescens CHA0Grows on C12-C32 alkanes 47
    P. fluorescens KOB2 alkB knockout mutant of CHA0, KmrThis study
    P. fluorescens KOB2Δ1 alkB knockout mutant of CHA0This study
    P. putida GPo12GPo1 cured of the OCT plasmid 25
    P. rugosa NRRL B-2295Grows on C12 22
Plasmids
    p2295 P. rugosa alkB fragment in pGEM7-Zf(+) 52
    pAF1 A. borkumensis AP1 alkG in pZEr0-2.1J. B. van Beilen
    pAP1J15.0-kb NcoI-EcoRV fragment of A. borkumensis AP1 in pZErO2.1, alkS alkB1This study
    pAP1S17.5-kb BglII fragment of A. borkumensis AP1 in pZErO2.1, alkGHJThis study
    pAP1T14.6-kb NheI fragment of A. borkumensis AP1 in pZErO2.1, ′alkSThis study
    pCHA0 P. fluorescens CHA0 alkB1 fragment in pGEM7-Zf(+) 43
    pCK217pUC18Sfi containing res-npt-res 27
    pCom5Expression vector with PalkB, Gmr 44
    pCom7Expression vector with PalkB, GmroriT 44
    pCom7B1 (PAO1) P. aeruginosa PAO1 alkB1 gene in pCom7This study
    pCom7B2 (PAO1) P. aeruginosa PAO1 alkB2 gene in pCom7This study
    pCom7B (GPo1) P. putida GPo1 alkB gene in pCom7This study
    pCom7alkB1 (AP1) A. borkumensis AP1 alkB1 gene in pCom7This study
    pCom7M (ADP1) Acinetobacter sp. ADP1 alkM gene in pCom7This study
    pCom8Broad-host-range expression vector with PalkB, GmroriT alkS 44
    pCom8B1 (PAO1) P. aeruginosa PAO1 alkB1 gene in pCom8This study
    pCom8B2 (PAO1) P. aeruginosa PAO1 alkB2 gene in pCom8This study
    pCom8B (GPo1) P. putida GPo1 alkB gene in pCom8This study
    pCom8M (ADP1) Acinetobacter sp. ADP1 alkM gene in pCom8This study
    pCom8MT (H37Rv) M. tuberculosis H37Rv alkB gene in pCom8This study
    pCom8B (2295) P. rugosa NRRL B-2295 alkB gene in pCom8This study
    pEX18TcGene replacement vector 19
    pEXPF1rnrB2pPF1rnrB PvuI fragment in pEX18TcThis study
    pGEc47 alkBFGHJKL alkST (GPo1) in pLAFR1 9
    pGEc47ΔBpGEc47, deletion in alkB 54
    pGEM7-Zf(+)Cloning vector, AprPromega
    pJMSB8Apr, PAI/04/03::parA in pJMSA8 27
    pKKPalk E. coli expression vector with PalkB, Apr 44
    pPF14.5-kb XhoI-BamHI fragment of P. fluorescens CHA0 in pGEM7-Zf(+), alkBThis study
    pPF1rnrBpPF1ΔSfiI-StuI, res-npt-res in alkBThis study
    pPF21 P. fluorescens CHA0 alkB gene in pCom5This study
    pPru13.0-kb SacI-BamHI fragment of P. rugosa NRRL B-2295 in pGEM7-Zf(+), alkBJ. B. van Beilen
    pSCYB11TcralkB rubA rubB (M. tuberculosis H37Rv) 34
    pSK2 A. borkumensis SK2 PCR fragment in pGEM7-Zf(+)J. B. van Beilen
    pUC18SfiCloning vector, Apr 16
    pUCP24 E. coli and Pseudomonas shuttle vector 60
    pUCPParAGmr, parA as EcoRI-HindIII fragment in pUCP24This study
    pZErO2.1Cloning vector, KmrInvitrogen