TABLE 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionReference or source
E. faecalis
    FA2-2rif fus17
    OG1Xstr gel33
    OG1SSstr spc25
    JH2SSstr spc49
    V583Clinical isolate (resistant to vancomycin, gentamicin, erythromycin, and tetracycline)45
    FA373FA2-2 carrying pAM373; Tn 918 (tet) on chromosome14
    FA3328Eep-negative mutant of OG1X3
    FA3333FA2-2 defective in cAD1This study
    FA3335FA2-2 with pAM3334 integrated in chromosomeThis study
    DS16Clinical isolate carrying pAD1 and pAD217
    39-5Clinical isolate carrying pPD153
E. coli DH5αFφ80 lacZΔM15 endA1 recA1 hsdR17 (rK mk+) supE44 thi-1 ΔgyrA96lacZYA-argF) U169Promega Research Laboratories
Plasmids
    pAM211pAD1::Tn 916 (tet)27
    pAM714pAD1::Tn 91731
    pAM4110pAM373::Tn917-lac18
    pCF10Carries Tn 925 (tet); responds to cCF1021
    pAM88pSU18 with additional (gram-positive) cat determinantVictoria Francia
    pAM401E. coli-E. faecalis shuttle vector; cat tet51
    pVA891E. coli plasmid vector; erm cat39
    pAM3332PCR fragment with part of cad (V583) in pVA891This study
    pAM3334PCR fragment of cad (internal) in pAM88This study