TABLE 1.

Plasmids and strains used in this study

Plasmid, strain, or genotypeDescriptionReference
pRKlac290RK2 vector for making lacZ transcriptional fusions9
Ps-lacZPs promoter in pRKlac290 (pGM976)23
PdnaA-lacZdnaA promoter from −369 to −1 in pRKlac290This study
dnaA′-lacZdnaA gene from −369 to +163 in pRKlac290This study
pLC17dnaA′-lacZ with DnaA box TTATCCAAG changed to TGAGCGCAGThis study
PxylX-dnaA′-lacZXylose-inducible promoter followed by dnaA gene from +1 to +140 in pRKlac290This study
PxylX-lacZXylose-inducible promoter in pRKlac290This study
pLC43E. coli dnaA gene from −190 to +151 in pRKlac290This study
pLC44E. coli dnaA gene from −190 to −1 in pRKlac290This study
PdnaA-dnaAPdnaA-lacZ with dnaA coding sequence replacing lacZThis study
CB15NWild-type synchronizable C. crescentus8
KCK116CB15N ΔssrA (in-frame deletion)17
LC202MG1655 ΔlacX74 ΔssrA::nptIIThis study